TGF beta inhibition enhances the generation of hematopoietic progenitors   from human ES cell-derived hemogenic endothelial cells using a stepwise   strategy

doi:10.1038/cr.2011.138

CORC > 北京大学 > 生命科学学院

	TGF beta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy
	Wang, Chengyan ; Tang, Xuming ; Sun, Xiaomeng ; Miao, Zhenchuan ; Lv, Yaxin ; Yang, Yanlei ; Zhang, Huidan ; Zhang, Pengbo ; Liu, Yang ; Du, Liying ; Gao, Yang ; Yin, Ming ; Ding, Mingxiao ; Deng, Hongkui
刊名	细胞研究英文版
	2012
关键词	embryonic stem cells hematopoietic progenitors SB431542 EMBRYONIC STEM-CELLS AORTIC ENDOTHELIUM MOUSE EMBRYO YOLK-SAC DIFFERENTIATION CULTURES STROMAL CELLS IPS CELLS BLOOD PRECURSORS PLACENTA
DOI	10.1038/cr.2011.138
英文摘要	Embryonic hematopoiesis is a complex process. Elucidating the mechanism regulating hematopoietic differentiation from pluripotent stem cells would allow us to establish a strategy to efficiently generate hematopoietic cells. However, the mechanism governing the generation of hematopoietic progenitors from human embryonic stem cells (hESCs) remains unknown. Here, on the basis of the emergence of CD43(+) hematopoietic cells from hemogenic endothelial (HE) cells, we demonstrated that VEGF was essential and sufficient, and that bFGF was synergistic with VEGF to specify the HE cells and the subsequent transition into CD43(+) hematopoietic cells. Significantly, we identified TGF beta as a novel signal to regulate hematopoietic development, as the TGF beta inhibitor SB 431542 significantly promoted the transition from HE cells into CD43(+) hematopoietic progenitor cells (HPCs) during hESC differentiation. By defining these critical signaling factors during hematopoietic differentiation, we can efficiently generate HPCs from hESCs. Our strategy could offer an in vitro model to study early human hematopoietic development.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299312900019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 31; ARTICLE; 1; 194-207; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/343552]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Chengyan,Tang, Xuming,Sun, Xiaomeng,et al. TGF beta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy[J]. 细胞研究英文版,2012.
APA	Wang, Chengyan.,Tang, Xuming.,Sun, Xiaomeng.,Miao, Zhenchuan.,Lv, Yaxin.,...&Deng, Hongkui.(2012).TGF beta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.细胞研究英文版.
MLA	Wang, Chengyan,et al."TGF beta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy".细胞研究英文版 (2012).