Negative Regulation of RIG-I-Mediated Innate Antiviral Signaling by   SEC14L1

doi:10.1128/JVI.01073-13

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	Negative Regulation of RIG-I-Mediated Innate Antiviral Signaling by SEC14L1
	Li, Meng-Tong ; Di, Wei ; Xu, Hao ; Yang, Yong-Kang ; Chen, Hai-Wei ; Zhang, Fei-Xiong ; Zhai, Zhong-He ; Chen, Dan-Ying
刊名	病毒学杂志
	2013
关键词	E3 UBIQUITIN LIGASE IMMUNE-RESPONSE ADAPTER PROTEIN RNA VIRUS RECOGNITION ACTIVATION DOMAIN MDA5 PHOSPHORYLATION
DOI	10.1128/JVI.01073-13
英文摘要	Retinoic acid-inducible gene I (RIG-I) is a key sensor for recognizing nucleic acids derived from RNA viruses and triggers beta interferon (IFN-beta) production. Because of its important role in antiviral innate immunity, the activity of RIG-I must be tightly controlled. Here, we used yeast two-hybrid screening to identify a SEC14 family member, SEC14L1, as a RIG-I-associated negative regulator. Transfected SEC14L1 interacted with RIG-I, and endogenous SEC14L1 associated with RIG-I in a viral infection-inducible manner. Overexpression of SEC14L1 inhibited transcriptional activity of the IFN-beta promoter induced by RIG-I but not TANK-binding kinase 1 (TBK1) and interferon regulatory factor 3 (IRF3). Knockdown of endogenous SEC14L1 in both HEK293T cells and HT1080 cells potentiated RIG-I and Sendai virus-triggered IFN-beta production as well as attenuated the replication of Newcastle disease virus. SEC14L1 interacted with the N-terminal domain of RIG-I (RIG-I caspase activation and recruitment domain [RIG-I-CARD]) and competed with VISA/MAVS/IPS-1/Cardif for RIG-I-CARD binding. Domain mapping further indicated that the PRELI-MSF1 and CRAL-TRIO domains but not the GOLD domain of SEC14L1 are required for interaction and inhibitory function. These findings suggest that SEC14L1; Virology; SCI(E); PubMed; 3; ARTICLE; 18; 10037-10046; 87
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342825]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Meng-Tong,Di, Wei,Xu, Hao,et al. Negative Regulation of RIG-I-Mediated Innate Antiviral Signaling by SEC14L1[J]. 病毒学杂志,2013.
APA	Li, Meng-Tong.,Di, Wei.,Xu, Hao.,Yang, Yong-Kang.,Chen, Hai-Wei.,...&Chen, Dan-Ying.(2013).Negative Regulation of RIG-I-Mediated Innate Antiviral Signaling by SEC14L1.病毒学杂志.
MLA	Li, Meng-Tong,et al."Negative Regulation of RIG-I-Mediated Innate Antiviral Signaling by SEC14L1".病毒学杂志 (2013).