Generation and characterization of Arabidopsis T-DNA insertion mutants

doi:10.1007/978-1-62703-580-4_13

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	Generation and characterization of Arabidopsis T-DNA insertion mutants
	Qu Li-Jia ; Qin Genji
刊名	methods in molecular biology clifton n j
	2014
DOI	10.1007/978-1-62703-580-4_13
英文摘要	Transfer DNA (T-DNA) insertion mutants are often used in forward and reverse genetics to reveal the molecular mechanisms of a particular biological process in plants. To generate T-DNA insertion mutants, T-DNA must be inserted randomly in the genome through transformation mediated by Agrobacterium tumefaciens. During generation of a T-DNA insertion mutant, Agrobacterium competent cells are first prepared and plasmids containing the T-DNA introduced into Agrobacterium cells. Agrobacterium containing T-DNA vectors are then used to transform T-DNA into Arabidopsis. After screening and identifying T-DNA insertion mutants with interesting phenotypes, genomic DNA is extracted from the mutants and used to isolate the T-DNA flanking sequences. To finally determine the mutated genes causing the specific phenotype in the T-DNA insertion mutants, cosegregation analysis and complementation or recapitulation analysis are needed. In this chapter, we describe detailed protocols for generation and characterization of T-DNA insertion mutants.; PubMed; 0; 241-58; 1062
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342605]
专题	生命科学学院
推荐引用方式 GB/T 7714	Qu Li-Jia,Qin Genji. Generation and characterization of Arabidopsis T-DNA insertion mutants[J]. methods in molecular biology clifton n j,2014.
APA	Qu Li-Jia,&Qin Genji.(2014).Generation and characterization of Arabidopsis T-DNA insertion mutants.methods in molecular biology clifton n j.
MLA	Qu Li-Jia,et al."Generation and characterization of Arabidopsis T-DNA insertion mutants".methods in molecular biology clifton n j (2014).