Nucleophosmin/B23 is a proliferate shuttle protein associated with   nuclear matrix

doi:10.1002/jcb.10706

CORC > 北京大学 > 生命科学学院

	Nucleophosmin/B23 is a proliferate shuttle protein associated with nuclear matrix
	Yun, JP ; Chew, EC ; Liew, CT ; Chan, JYH ; Jin, ML ; Ding, MX ; Fai, YH ; Li, HKR ; Liang, XM ; Wu, QL
刊名	细胞生物化学杂志
	2003
关键词	nucleophosmin (NPM)/B23 liver regeneration partial hepatectomy nuclear matrix immunohistochemistry two-dimensional gel electrophoresis MAJOR NUCLEOLAR PROTEINS CENTROSOME DUPLICATION BINDING-ACTIVITY NUMATRIN B23 CELL-CYCLE IDENTIFICATION TRANSLOCATION EXPRESSION
DOI	10.1002/jcb.10706
英文摘要	It has become obvious that a better understanding and potential elucidation of the nucleolar phosphoprotein B23 involving in functional interrelationship between nuclear organization and gene expression. In present study, protein B23 expression were investigated in the regenerative hepatocytes at different periods (at days 0, 1,2, 3, 4, 7) during liver regeneration after partial hepatectomy on the rats with immunohistochemistry and Western blot analysis. Another experiment was done with immunolabeling methods and two-dimensional (2-D) gel electrophoresis for identification of B23 in the regenerating hepatocytes and HepG2 cells (hepatoblastoma cell line) after sequential extraction with detergents, nuclease, and salt. The results showed that its expression in the hepatocytes had a locative move and quantitative change during the process of liver regeneration post-operation. Its immunochemical localization in the hepatocytes during the process showed that it moved from nucleoli of the hepatocytes in the stationary stage to nucleoplasm, cytoplasm, mitotic spindles, and mitotic chromosomes of the hepatocytes in the regenerating livers. it was quantitatively increased progressively to peak level at day 3 post-operation and declined gradually to normal level at day 7. It was detected in nuclear matrix protein (NMP) composition extracted from the regenerating hepatocytes and HepG2 cells and identified with isoelectric point (pl) value of 5.1 and molecular weight of 40 kDa. These results indicated that B23 was a proliferate shuttle protein involving in cell cycle and cell proliferation associated with nuclear matrix. (C) 2003 Wiley-Liss, Inc.; Biochemistry & Molecular Biology; Cell Biology; SCI(E); 35; ARTICLE; 6; 1140-1148; 90
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/257955]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yun, JP,Chew, EC,Liew, CT,et al. Nucleophosmin/B23 is a proliferate shuttle protein associated with nuclear matrix[J]. 细胞生物化学杂志,2003.
APA	Yun, JP.,Chew, EC.,Liew, CT.,Chan, JYH.,Jin, ML.,...&Wu, QL.(2003).Nucleophosmin/B23 is a proliferate shuttle protein associated with nuclear matrix.细胞生物化学杂志.
MLA	Yun, JP,et al."Nucleophosmin/B23 is a proliferate shuttle protein associated with nuclear matrix".细胞生物化学杂志 (2003).