Purification and characterization of a new peptide with analgesic effect   from the scorpion Buthus martensi Karch

doi:10.1111/j.1399-3011.2004.00164.x

CORC > 北京大学 > 生命科学学院

	Purification and characterization of a new peptide with analgesic effect from the scorpion Buthus martensi Karch
	Cao, ZY ; Mi, ZM ; Cheng, GF ; Shen, WQ ; Xiao, X ; Liu, XM ; Liang, XT ; Yu, DQ
刊名	journal of peptide research
	2004
关键词	analgesic effect BmK AngM1 Buthus martensi Karch electrophysiology CENTRUROIDES-NOXIUS HOFFMANN ANDROCTONUS-AUSTRALIS-HECTOR MOLECULAR CHARACTERIZATION INSECT NEUROTOXIN SODIUM-CHANNELS RECEPTOR-SITES KARSCH TOXIN BINDING VENOM
DOI	10.1111/j.1399-3011.2004.00164.x
英文摘要	A new peptide, designated as Buthus martensi Karch (BmK) AngM1, with an isoelectric point (pI) of 5.8 was purified and characterized from the venom of Buthus martensi Karch. The molecular mass was calculated as 7040.5 Da from multiple-charged ions by elelctrospray ionization mass spectroscopy (ESI/MS). The complete amino acid sequence of BmK AngM1 of 64 amino acid residues was determined by automatic sequencing of N-terminal part of the native peptide and the fragments of reduced and S-carboxymethylated (RCM)-peptide degraded by Staphylococcus aureaus V-8 protease and TPCK(N-p-Tosyl-L-phenylalanine chloromethyl ketone)-treated trypsin. Bioactivity tested using mouse-twisting model showed an evident analgesic effect with 63.0% (P < 0.001) inhibition efficiency at the dose of 0.8 mg/kg, but the LD50 was larger than 50 mg/kg. Electrophysiological studies showed that BmK AngM1 at the concentration of 1 muM obviously inhibit voltage-dependent Na+ current (I-Na) and voltage-dependent delayed rectifier K+ current (I-K) but had no effects on transient K+ current.; Biochemical Research Methods; Biochemistry & Molecular Biology; SCI(E); 0; ARTICLE; 1; 33-41; 64
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/254878]
专题	生命科学学院
推荐引用方式 GB/T 7714	Cao, ZY,Mi, ZM,Cheng, GF,et al. Purification and characterization of a new peptide with analgesic effect from the scorpion Buthus martensi Karch[J]. journal of peptide research,2004.
APA	Cao, ZY.,Mi, ZM.,Cheng, GF.,Shen, WQ.,Xiao, X.,...&Yu, DQ.(2004).Purification and characterization of a new peptide with analgesic effect from the scorpion Buthus martensi Karch.journal of peptide research.
MLA	Cao, ZY,et al."Purification and characterization of a new peptide with analgesic effect from the scorpion Buthus martensi Karch".journal of peptide research (2004).