JZTX-IV, a unique acidic sodium channel toxin isolated from the spider   Chilobrachys jingzhao

doi:10.1016/j.toxicon.2008.08.018

CORC > 北京大学 > 生命科学学院

	JZTX-IV, a unique acidic sodium channel toxin isolated from the spider Chilobrachys jingzhao
	Wang, Meichi ; Diao, Jianbo ; Li, Jiang ; Tang, Jianzhou ; Lin, Yin ; Hu, Weijun ; Zhang, Yongqun ; Xiao, Yucheng ; Liang, Songping
刊名	toxicon
	2008
关键词	JZTX-IV TTX-R TTX-S Sodium channel DRG Cardiac myocyte GATED NA+ CHANNELS GATING MODIFIER SELENOCOSMIA-HUWENA BINDING NEUROTOXINS ACTIVATION DIVERSITY SEQUENCE VENOM
DOI	10.1016/j.toxicon.2008.08.018
英文摘要	Neurotoxins are important tools to explore the structure and function relationship of different ion channels. From the venom of Chinese spider Chilobrachys jingzhao, a novel toxin, Jingzhaotoxin-IV (JZTX-IV), is isolated and characterized. It consists of 34 amino acid residues including six acidic residues clustered with negative charge (pI = 4.29). The full-length cDNA of JZTX-IV encodes an 86-amino acid precursor containing a signal peptide of 21 residues, a mature peptide of 34 residues and an intervening sequence of 29 residues with terminal Lys-Gly as the signal of amidation. Under whole-cell patch clamp conditions, JZTX-IV inhibits Current and slows the inactivation of sodium channels by shifting the voltage dependence of activation to more depolarized potentials on DRG neurons, therefore, differs from the classic site 4 toxins that shift voltage dependence of activation in the opposite direction. In addition, JZTX-IV shows a slowing inactivation of sodium channel with a hyperpolarizing shift of the steady-state inactivation on acutely isolated rat cardiac cell and DRG neurons, differs from the classic site 3 toxins that do not affect the steady-state of inactivation. At high concentration, JZTX-IV has no significant effect on tetrodotoxin-resistant (TTX-R) sodium channels on rat DRG neurons and tetrodotoxin-sensitive (TTX-S) sodium channels on hippocampal neurons. Our data establish that, contrary to known toxins, JZTX-IV neither binds to the previously characterized classic site 4, nor site 3 by modifying channel gating, thus making it a novel probe of channel gating in sodium channels with potential to shed new light on this process. (C) 2008 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000262053700006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Pharmacology & Pharmacy; Toxicology; SCI(E); 7; ARTICLE; 8; 871-880; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/247685]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Meichi,Diao, Jianbo,Li, Jiang,et al. JZTX-IV, a unique acidic sodium channel toxin isolated from the spider Chilobrachys jingzhao[J]. toxicon,2008.
APA	Wang, Meichi.,Diao, Jianbo.,Li, Jiang.,Tang, Jianzhou.,Lin, Yin.,...&Liang, Songping.(2008).JZTX-IV, a unique acidic sodium channel toxin isolated from the spider Chilobrachys jingzhao.toxicon.
MLA	Wang, Meichi,et al."JZTX-IV, a unique acidic sodium channel toxin isolated from the spider Chilobrachys jingzhao".toxicon (2008).