Inorganic binding peptide-mediated immobilization based on baculovirus   surface display system

doi:10.1002/jobm.200900359

CORC > 北京大学 > 生命科学学院

	Inorganic binding peptide-mediated immobilization based on baculovirus surface display system
	Song, Lei ; Liu, Yingying ; Chen, Jinchun
刊名	journal of basic microbiology
	2010
关键词	Baculovirus surface display Inorganic binding peptide Genetic recombination Bio-inorganic composite GENETICALLY-ENGINEERED VIRUSES NUCLEAR POLYHEDROSIS-VIRUS ZINC-OXIDE PROTEINS NANOPARTICLES POLYPEPTIDES ENVELOPE GENE BIOMINERALIZATION GLYCOPROTEIN
DOI	10.1002/jobm.200900359
英文摘要	The biomolecule-mediated assembly of novel composites has been the subject of numerous investigations during the last years, providing new insights into material science and engineering. Via molecular biology technology, we were able to introduce the genetically engineered polypeptide for inorganics (GEPI) as a molecular binder into biomolecules such as phage viruses, to assemble hybrid functional nanoarchitectures. In the present work, we introduced a novel nanocomposite comprising the Autographa californica nuclear polyhedrosis virus (AcNPV) and nanoparticles bound to it. Our results show that a GEPI-encoding gene was successfully introduced by recombination into a eukaryotic expression bacmid and finally displayed outside of the AcNPV after transfection into Sf9 insect cells using the Bac-to-Bac baculovirus expression system. The recombinant baculovirus maintained both the viral infectivity and the specific binding activity of the GEPI. The construction of the gene in the recombinant plasmid was examined by polymerase chain reaction analysis and enzymatic digestion identification, and verified by gene sequencing. Surface display of the fused peptide was revealed by Western blot analysis in dissolution studies and determined by immunogold electron microscopy. Adherence of nanoparticles to the recombinant baculovirus was visualized by transmission electron microscopy analysis. Here, we demonstrated the possibilities of combining peptide-mediated immobilization with baculovirus surface display technology.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000283103600007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Microbiology; SCI(E); 1; ARTICLE; 5; 457-464; 50
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/242338]
专题	生命科学学院
推荐引用方式 GB/T 7714	Song, Lei,Liu, Yingying,Chen, Jinchun. Inorganic binding peptide-mediated immobilization based on baculovirus surface display system[J]. journal of basic microbiology,2010.
APA	Song, Lei,Liu, Yingying,&Chen, Jinchun.(2010).Inorganic binding peptide-mediated immobilization based on baculovirus surface display system.journal of basic microbiology.
MLA	Song, Lei,et al."Inorganic binding peptide-mediated immobilization based on baculovirus surface display system".journal of basic microbiology (2010).