microRNA expression in hepatitis B virus infected primary treeshrew   hepatocytes and the independence of intracellular miR-122 level for de   novo HBV infection in culture

doi:10.1016/j.viro1.2013.10.016

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	microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture
	Xu, Guangwei ; Gao, Zhenchao ; He, Wenhui ; Ma, Yanting ; Feng, Xiaofeng ; Cai, Tao ; Lu, Fengmin ; Liu, Li ; Li, Wenhui
刊名	virology
	2014
关键词	Hepatitis B virus MicroRNA-122 Tupaia Hepatocyte RNA deep sequencing MicroRNA database Sodium taurocholate cotransporting polypeptide (NTCP) De novo infection Pathogenesis LIVER-SPECIFIC MICRORNA TUPAIA HEPATOCYTES REPLICATION IDENTIFICATION SUPPRESSION DNA
DOI	10.1016/j.viro1.2013.10.016
英文摘要	Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2-NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest that de novo HBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells. (C) 2013 Elsevier Inc. All rights reserved.; Virology; SCI(E); 2; ARTICLE; liwenhui@nibs.ac.cn; 247-254; 448
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/216858]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Guangwei,Gao, Zhenchao,He, Wenhui,et al. microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture[J]. virology,2014.
APA	Xu, Guangwei.,Gao, Zhenchao.,He, Wenhui.,Ma, Yanting.,Feng, Xiaofeng.,...&Li, Wenhui.(2014).microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture.virology.
MLA	Xu, Guangwei,et al."microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture".virology (2014).