Three-dimensional analysis of the 16 nm urothelial plaque particle:   Luminal surface exposure, preferential head-to-head interaction, and   hinge formation

doi:10.1006/jmbi.1998.2304

CORC > 北京大学 > 生命科学学院

	Three-dimensional analysis of the 16 nm urothelial plaque particle: Luminal surface exposure, preferential head-to-head interaction, and hinge formation
	Kachar, B ; Liang, FX ; Lins, U ; Ding, MX ; Wu, XR ; Stoffler, D ; Aebi, U ; Sun, TT
刊名	分子生物学杂志
	1999
关键词	bladder epithelium asymmetric unit membrane uroplakin urothelial plaque urothelial differentiation MAMMALIAN URINARY-BLADDER ASYMMETRIC UNIT MEMBRANE TRANSITIONAL EPITHELIUM 3-DIMENSIONAL STRUCTURE PLASMA-MEMBRANE APICAL MEMBRANE CELL-SURFACE DIFFERENTIATION UROPLAKIN VESICLES
DOI	10.1006/jmbi.1998.2304
英文摘要	The luminal surface of mouse urothelium in contact with the urine is almost entirely covered with plaques consisting of uroplakin-containing particles that form p6 hexagonal crystals with a center-to-center distance of 16 nm. A combination of quick-freeze/deep-etch images and our previous negative staining data indicate that the head domain of the uroplakin particle, which is exposed without an extensive glycocalyx shield, interacts closely with the head domains of the neighboring particles, while the membrane-embedded tail domains are farther apart; and that urothelial particles and plaques are not rigid structures as they can change their configuration in response to mechanical perturbations. Based on these data, we have constructed three-dimensional models depicting the structural organization of urothelial particles and plaques. Our models suggest that the head-to-head interaction may play a key role in determining the shape and size of the urothelial plaques. These models can explain many properties of urothelial plaques including their unique shape, detergent-insolubility, and morphological changes during vesicle maturation. (C) 1999 Academic Press.; Biochemistry & Molecular Biology; SCI(E); 50; ARTICLE; 2; 595-608; 285
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/214721]
专题	生命科学学院
推荐引用方式 GB/T 7714	Kachar, B,Liang, FX,Lins, U,et al. Three-dimensional analysis of the 16 nm urothelial plaque particle: Luminal surface exposure, preferential head-to-head interaction, and hinge formation[J]. 分子生物学杂志,1999.
APA	Kachar, B.,Liang, FX.,Lins, U.,Ding, MX.,Wu, XR.,...&Sun, TT.(1999).Three-dimensional analysis of the 16 nm urothelial plaque particle: Luminal surface exposure, preferential head-to-head interaction, and hinge formation.分子生物学杂志.
MLA	Kachar, B,et al."Three-dimensional analysis of the 16 nm urothelial plaque particle: Luminal surface exposure, preferential head-to-head interaction, and hinge formation".分子生物学杂志 (1999).