PEG介导下香菇的转化

doi:10.3321/j.issn:1672-9072.2001.10.018

CORC > 北京大学 > 生命科学学院

	PEG介导下香菇的转化; PEG-mediated Transformation of Lentinus edodes
	孙丽 ; 许伟宏 ; 蔡华清 ; 胡鸢雷 ; 林忠平
刊名	植物学报
	2001
关键词	香菇转化三磷酸甘油醛脱氢酶基因启动子 gus PEG
DOI	10.3321/j.issn:1672-9072.2001.10.018
英文摘要	表达载体p301-bG1含有香菇(Lentinus edodes(Berk.)Sing)三磷酸甘油醛脱氢酶启动子驱动下的gus基因和除草剂抗性基因.利用PEG法实现了p301-bG1对香菇原生质体的转化.香菇原生质体与经PEG纯化的质粒DNA混合,用PEG处理后培养于含40μg/mL除草剂的CYM再生平板上,得到了抗除草剂和有GUS活性的转化菌株.虽然这种方法转化效率较低,但不需要昂贵的仪器和限制性内切酶,为蘑菇的分子育种研究提供了一种简便经济的转化方法.; Expression vector p301-bG1 contains a gus gene and a bialaphos resistance gene both driven by glyceraldehydes-3-phosphate dehydrogenase (GPD) gene promoter isolated from Lentinus edodes (Berk.)Sing. Using p301-bG1, PEG-mediated transformation of protoplast of L. edodes was studied. Mixed with PEG-purified plasmid DNA, the protoplasts of L. edodes were treated with PEG solution and cultured on CYM regeneration plate containing 40 μg/mL bialaphos. Bialaphos-resistant and GUS-positive transformants were obtained using this transformation system. Although the transformation efficiency was relatively low, the proto cols release large expenses on expensive instrument and restriction enzymes, providing a simple and economical method for mushroom breeding at the molecular level.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000172013400018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中国科学引文数据库(CSCD); 2; 10; 1089-1092; 43
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/211332]
专题	生命科学学院
推荐引用方式 GB/T 7714	孙丽,许伟宏,蔡华清,等. PEG介导下香菇的转化, PEG-mediated Transformation of Lentinus edodes[J]. 植物学报,2001.
APA	孙丽,许伟宏,蔡华清,胡鸢雷,&林忠平.(2001).PEG介导下香菇的转化.植物学报.
MLA	孙丽,et al."PEG介导下香菇的转化".植物学报 (2001).