A draft sequence of the rice (Oryza sativa ssp indica) genome

doi:10.1007/BF02901901

CORC > 北京大学 > 生命科学学院

	A draft sequence of the rice (Oryza sativa ssp indica) genome
	Yu, J ; Hu, SN ; Wang, J ; Li, SG ; Wong, KSG ; Liu, B ; Deng, Y ; Dai, L ; Zhou, Y ; Zhang, XQ ; Cao, ML ; Liu, J ; Sun, JD ; Tang, JB ; Chen, YJ ; Huang, XB ; Lin, W ; Ye, C ; Tong, W ; Cong, LJ ; Geng, JN ; Han, YJ ; Li, L ; Li, W ; Hu, GQ ; Huang, XG ; Li, WJ ; Li, J ; Liu, ZW ; Li, L ; Liu, JP ; Qi, QH ; Liu, JS ; Li, L ; Wang, XG ; Lu, H ; Wu, TT ; Zhu, M ; Ni, PX ; Han, H ; Dong, W ; Ren, XY ; Feng, XL ; Cui, P ; Li, XR ; Wang, H ; Xu, X ; Zhai, WX ; Xu, Z ; Zhang, JS ; He, SJ ; Zhang, JG ; Xu, JC ; Zhang, KL ; Zheng, XW ; Dong, JH ; Zeng, WY ; Tao, L ; Chen, XW ; He, J ; Liu, DF ; Tian, W ; Tian, CG ; Xia, HG ; Li, G ; Gao, H
刊名	科学通报英文版
	2001
关键词	rice genome draft sequences DNA CONSTRUCTION LIBRARY
DOI	10.1007/BF02901901
英文摘要	The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have produced a draft rice genome sequence of Oryza sativa ssp. indica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEMBLER, a custom-designed software package used for the initial assembly, were verified rigorously by comparisons against finished BAC clone sequences from both indica and japanica strains, available from the public databases. Over all, 96.3% of full-length cDNAs, 96.4% of STS, STR, RFLP markers, 94.0% of ESTs and 94.9% unigene clusters were identified from the draft sequence. Our preliminary analysis on the data set shows that our rice draft sequence is consistent with the comman standard accepted by the genome sequencing community. The unconditional release of the draft to the public also undoubtedly provides a fundamental resource to the international scientific communities to facilitate genomic and genetic studies on rice biology.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000173381900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 20; ARTICLE; 23; 1937-1942; 46
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/210467]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yu, J,Hu, SN,Wang, J,et al. A draft sequence of the rice (Oryza sativa ssp indica) genome[J]. 科学通报英文版,2001.
APA	Yu, J.,Hu, SN.,Wang, J.,Li, SG.,Wong, KSG.,...&Gao, H.(2001).A draft sequence of the rice (Oryza sativa ssp indica) genome.科学通报英文版.
MLA	Yu, J,et al."A draft sequence of the rice (Oryza sativa ssp indica) genome".科学通报英文版 (2001).