A 3 center dot 1-kb genomic fragment of Bacillus subtilis encodes the   protein inhibiting growth of Xanthomonas oryzae pv. oryzae

doi:10.1046/j.1365-2672.2001.01475.x

CORC > 北京大学 > 生命科学学院

	A 3 center dot 1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae
	Lin, D ; Qu, LJ ; Gu, H ; Chen, Z
刊名	journal of applied microbiology
	2001
关键词	GRAM-POSITIVE BACTERIA DISEASE RESISTANCE ANTIBACTERIAL PEPTIDE MOLECULAR-CLONING GENE-EXPRESSION BOMBYX-MORI RICE BLIGHT ANTIBIOTICS DEFENSINS
DOI	10.1046/j.1365-2672.2001.01475.x
英文摘要	Aims: To clone genes of Bacillus subtilis encoding peptides that inhibit the growth of Xanthomonas orzae pv. oryzae (Xoo). Methods and Results: A 3.1-kb DNA fragment from B. subtilis SO 113 encoding peptides that inhibit the growth of Xoo (anti-Xoo, showing an inhibition zone) was isolated from a plasmid library of B. subtilis 6 GM15. Sequence analysis revealed that it contained three complete open reading frames (ORFs): ybcO, ybcS and a novel ORF designated vbcPQ Deleting the last 96 bp of ybcS from the plasmid eliminated the anti-Xoo activity, suggesting that ybcS is required for producing the anti-Xoo activity. However, no anti-Xoo activity could be detected for the plasmid with ybcS alone. Further analysis showed that ybcO, at least, was also required to obtain the anti-Xoo activity. Conclusions: A fragment of B. subtilis has been cloned that expresses an anti-Xoo activity that requires ybcS and ybcO. Significance and Impact of the Study: These genes could be useful for the genetic engineering of resistance to rice bacterial diseases and for the design of new anti-Xoo biocontrol agents.; Biotechnology & Applied Microbiology; Microbiology; SCI(E); PubMed; 0; ARTICLE; 6; 1044-1050; 91
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200491]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lin, D,Qu, LJ,Gu, H,et al. A 3 center dot 1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae[J]. journal of applied microbiology,2001.
APA	Lin, D,Qu, LJ,Gu, H,&Chen, Z.(2001).A 3 center dot 1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae.journal of applied microbiology.
MLA	Lin, D,et al."A 3 center dot 1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae".journal of applied microbiology (2001).