Expression and purification of Huwentoxin-I in baculovirus system

doi:10.1016/j.pep.2005.02.012

CORC > 北京大学 > 生命科学学院

	Expression and purification of Huwentoxin-I in baculovirus system
	Ji, WJ ; Zhang, XY ; Hu, HC ; Chen, JY ; Gao, Y ; Liang, SP ; An, CC
刊名	蛋白质表达与纯化
	2005
关键词	Huwentoxin-I nickel-chelating affinity chromatography baculovirus expression system SPIDER SELENOCOSMIA-HUWENA NEUROTOXIN SEQUENCE VENOM
DOI	10.1016/j.pep.2005.02.012
英文摘要	Huwentoxin-I (HWTX-I) is a novel neurotoxin isolated from the venom of Orinithoctonus huwena. Based on its biological activity, HWTX-I could be developed as a pain-killer for clinical purpose. Production of HWTX-I by the bacterium or yeast expression systems resulted in poor yields and the purified protein was proved to have lower biological activity than that of native one. So, for the first time, we introduced a new method to express HWTX-I gene in Sf9 cells using baculovirus expression system. Recombinant HWTX-I was recognized by Western blotting and then purified by nickel-chelating affinity chromatography under native conditions. Recombinant HWTX-I showed identical amino acid sequence as native form and exhibited similar effect on muscular transmission with that of native form. These results indicate that the baculovirus expression system and native purification strategy are viable ways to produce active HWTX-I. (c) 2005 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000229192800028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; SCI(E); PubMed; 7; ARTICLE; 2; 454-458; 41
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199762]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ji, WJ,Zhang, XY,Hu, HC,et al. Expression and purification of Huwentoxin-I in baculovirus system[J]. 蛋白质表达与纯化,2005.
APA	Ji, WJ.,Zhang, XY.,Hu, HC.,Chen, JY.,Gao, Y.,...&An, CC.(2005).Expression and purification of Huwentoxin-I in baculovirus system.蛋白质表达与纯化.
MLA	Ji, WJ,et al."Expression and purification of Huwentoxin-I in baculovirus system".蛋白质表达与纯化 (2005).