A novel chemical-defined medium with bFGF and N2B27 supplements supports   undifferentiated growth in human embryonic stem cells

doi:10.1016/j.bbrc.2006.05.086

CORC > 北京大学 > 生命科学学院

	A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells
	Liu, YX ; Song, ZH ; Zhao, Y ; Qin, H ; Cai, J ; Zhang, H ; Yu, TX ; Jiang, SM ; Wang, GW ; Ding, MX ; Deng, HK
刊名	生物化学与生物物理学研究通讯
	2006
关键词	human embryonic stem cells self-renewal pluripotency N2B27 bFGF chemical-defined medium long-term culture fibronectin HUMAN FEEDER CELLS MAINTAIN PLURIPOTENCY SELF-RENEWAL DIFFERENTIATION MAINTENANCE ABSENCE EXPRESSION CULTURE NOGGIN LINES
DOI	10.1016/j.bbrc.2006.05.086
英文摘要	Traditionally, undifferentiated human embryonic stem cells (hESCs) are maintained on mouse embryonic fibroblast (MEF) cells or on matrigel with an MEF-conditioned medium (CM), which hampers the clinical applications of hESCs due to the contamination by animal pathogens. Here we report a novel chemical-defined medium using DMEM/F12 supplemented with N2, B27, and basic fibroblast growth factor (bFGF) [termed NBF]. This medium can support prolonged self-renewal of hESCs. hESCs cultured in NBF maintain an undifferentiated state and normal karyotype, are able to form embryoid bodies in vitro, and differentiate into three germ layers and extraembryonic cells. Furthermore, we find that hESCs cultured in NBF possess a low apoptosis rate and a high proliferation rate compared with those cultured in MEF-CM. Our findings provide a novel, simplified chemical-defined culture medium suitable for further therapeutic applications and developmental studies of hESCs. (c) 2006 Elsevier Inc. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 65; ARTICLE; 1; 131-139; 346
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198843]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, YX,Song, ZH,Zhao, Y,et al. A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells[J]. 生物化学与生物物理学研究通讯,2006.
APA	Liu, YX.,Song, ZH.,Zhao, Y.,Qin, H.,Cai, J.,...&Deng, HK.(2006).A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells.生物化学与生物物理学研究通讯.
MLA	Liu, YX,et al."A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells".生物化学与生物物理学研究通讯 (2006).