Identification of genes regulated by Wnt/beta-catenin pathway and   involved in apoptosis via microarray analysis

doi:10.1186/1471-2407-6-221

CORC > 北京大学 > 生命科学学院

	Identification of genes regulated by Wnt/beta-catenin pathway and involved in apoptosis via microarray analysis
	Huang, Moli ; Wang, Yihua ; Sun, Daochun ; Zhu, Hongxia ; Yin, Yanbing ; Zhang, Wei ; Yang, Shangbin ; Quan, Lanping ; Bai, Jinfeng ; Wang, Shengqi ; Chen, Quan ; Li, Songgang ; Xu, Ningzhi
刊名	bmc cancer
	2006
关键词	PROSTATE-CANCER CELLS HUMAN COLORECTAL-CANCER WNT SIGNALING PATHWAYS NF-KAPPA-B BETA-CATENIN MONOCLONAL-ANTIBODY RNA INTERFERENCE TRANSCRIPTIONAL ACTIVATION INHIBITS APOPTOSIS TNF-ALPHA
DOI	10.1186/1471-2407-6-221
英文摘要	Background: Wnt/beta-catenin pathway has critical roles in development and oncogenesis. Although significant progress has been made in understanding the downstream signaling cascade of this pathway, little is known regarding Wnt/beta-catenin pathway modification of the cellular apoptosis. Methods: To identify potential genes regulated by Wnt/beta-catenin pathway and involved in apoptosis, we used a stably integrated, inducible RNA interference (RNAi) vector to specific inhibit the expression and the transcriptional activity of beta-catenin in HeLa cells. Meanwhile, we designed an oligonucleotide microarray covering 1384 apoptosis-related genes. Using oligonucleotide microarrays, a series of differential expression of genes was identified and further confirmed by RTPCR. Results: Stably integrated inducible RNAi vector could effectively suppress beta-catenin expression and the transcriptional activity of beta-catenin/TCF. Meanwhile, depletion of beta-catenin in this manner made the cells more sensitive to apoptosis. 130 genes involved in some important cell-apoptotic pathways, such as PTEN-PI3K-AKT pathway, NF-kappa B pathway and p53 pathway, showed significant alteration in their expression level after the knockdown of beta-catenin. Conclusion: Coupling RNAi knockdown with microarray and RT-PCR analyses proves to be a versatile strategy for identifying genes regulated by Wnt/ beta-catenin pathway and for a better understanding the role of this pathway in apoptosis. Some of the identified beta-catenin/TCF directed or indirected target genes may represent excellent targets to limit tumor growth.; Oncology; SCI(E); PubMed; 0; ARTICLE; 221; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198717]
专题	生命科学学院
推荐引用方式 GB/T 7714	Huang, Moli,Wang, Yihua,Sun, Daochun,et al. Identification of genes regulated by Wnt/beta-catenin pathway and involved in apoptosis via microarray analysis[J]. bmc cancer,2006.
APA	Huang, Moli.,Wang, Yihua.,Sun, Daochun.,Zhu, Hongxia.,Yin, Yanbing.,...&Xu, Ningzhi.(2006).Identification of genes regulated by Wnt/beta-catenin pathway and involved in apoptosis via microarray analysis.bmc cancer.
MLA	Huang, Moli,et al."Identification of genes regulated by Wnt/beta-catenin pathway and involved in apoptosis via microarray analysis".bmc cancer (2006).