Capillary electrophoresis and circular dichroism study of trichosanthin   and its mutants

doi:10.1016/S0039-9140(02)00046-2

CORC > 北京大学 > 生命科学学院

	Capillary electrophoresis and circular dichroism study of trichosanthin and its mutants
	Zhang, CY ; An, CC ; Wang, RY ; Gong, YX ; Ma, H ; Chen, DY ; Chen, ZL
刊名	talanta
	2002
关键词	circular dichroism trichosanthin capillary electrophoresis CHORIOCARCINOMA CELLS MECHANISM OXIDASE
DOI	10.1016/S0039-9140(02)00046-2
英文摘要	The type-I ribosome-inactivating protein trichosanthin (TCS) has a broad spectrum of biological and pharmacological activities, including abortifacient, anti-tumor and anti-human-immunodeficiency-virus (anti-HrV). In this study, circular dichroism (CD) and capillary electrophoresis were used for the first time to study TCS and its two TCS mutants of Y55G TCS (tyrosine 55 converted to glycine) and FYY140-142GSA TCS (tripeptide phenylalanine-tyrosine-tyrosine 140-142 converted to glycine-serine-alanine). The results indicated that the substitution of amino acids changed the secondary structures and the hydrophobility of TCS. Moreover, both Y55G TCS and FYY140-142GSA TCS demonstrated attenuated cytotoxicity and reactive oxygen species (ROS) production in human choriocarcinoma cells (JAR cells) as compared to natural TCS and wild-type TCS. Our results demonstrated the cytotoxicity of TCS on JAR cells and TCS-induced production of ROS might be TCS-conformational related, suggesting that CD and capillary electrophoresis study might throw new insight into the anti-tumor and anti-HIV mechanism of TCS. (C) 2002 Elsevier Science B.V. All rights reserved.; Chemistry, Analytical; SCI(E); EI; PubMed; 5; ARTICLE; 3; 467-473; 57
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/197731]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, CY,An, CC,Wang, RY,et al. Capillary electrophoresis and circular dichroism study of trichosanthin and its mutants[J]. talanta,2002.
APA	Zhang, CY.,An, CC.,Wang, RY.,Gong, YX.,Ma, H.,...&Chen, ZL.(2002).Capillary electrophoresis and circular dichroism study of trichosanthin and its mutants.talanta.
MLA	Zhang, CY,et al."Capillary electrophoresis and circular dichroism study of trichosanthin and its mutants".talanta (2002).