Preliminary crystallographic studies of purine nucleoside phosphorylase   from the cariogenic pathogen Streptococcus mutans

doi:10.1107/S1744309109045059

CORC > 北京大学 > 生命科学学院

	Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans
	Hou, Qiao-Ming ; Liu, Xiang ; Brostromer, Erik ; Li, Lan-Fen ; Su, Xiao-Dong
刊名	acta crystallographica section f structural biology and crystallization communications
	2009
关键词	CRYSTAL-STRUCTURE RESOLUTION INHIBITOR COMPLEX ANALOG GENE
DOI	10.1107/S1744309109045059
英文摘要	The pun Lambda gene of the cariogenic pathogen Streptococcus mutans encodes purine nucleoside phosphorylase (PNP), which is a pivotal enzyme in the nucleotide-salvage pathway, catalyzing the phosphorolysis of purine nucleosides to generate purine bases and alpha-ribose 1-phosphate. In the present work, the PNP protein was expressed in Escherichia coli strain BL21 (DE3) in a soluble form at a high level. After purification of the PNP enzyme, the protein was crystallized using the sitting-drop vapour-diffusion technique; the crystals diffracted to 1.6 angstrom resolution at best. The crystals belonged to space group H3, with unit-cell parameters a = b = 113.0, c = 60.1 angstrom.; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 0; ARTICLE; Pt 12; 1289-1291; 65
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/197410]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hou, Qiao-Ming,Liu, Xiang,Brostromer, Erik,et al. Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans[J]. acta crystallographica section f structural biology and crystallization communications,2009.
APA	Hou, Qiao-Ming,Liu, Xiang,Brostromer, Erik,Li, Lan-Fen,&Su, Xiao-Dong.(2009).Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans.acta crystallographica section f structural biology and crystallization communications.
MLA	Hou, Qiao-Ming,et al."Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans".acta crystallographica section f structural biology and crystallization communications (2009).