Small heat shock protein AgsA forms dynamic fibrils

doi:10.1016/j.febslet.2011.09.042

CORC > 北京大学 > 生命科学学院

	Small heat shock protein AgsA forms dynamic fibrils
	Shi, Xiaodong ; Wang, Zhao ; Yan, Linxuan ; Ezemaduka, Anastasia N. ; Fan, Guizhen ; Wang, Rui ; Fu, Xinmiao ; Yin, Changcheng ; Chang, Zengyi
刊名	欧洲生化学会联合会快报
	2011
关键词	Small heat shock protein Chaperone Fibril Amyloid Electron microscopy Cryo-electron tomography CHAPERONE-LIKE ACTIVITY ESCHERICHIA-COLI BIOCHEMICAL-CHARACTERIZATION DENATURED PROTEIN CRYSTAL-STRUCTURE ALPHA-CRYSTALLIN AMYLOID FIBRILS IN-VITRO IBPB BINDING
DOI	10.1016/j.febslet.2011.09.042
英文摘要	As a class of molecular chaperones, small heat shock proteins (sHsps) usually exist as multi-subunit spherical oligomers. In this study, we report that AgsA, a sHsp of Salmonella enterica serovar Typhimurium, spontaneously forms fibrils in vitro. These fibrils tend to be formed at elevated temperature and do not share the characteristics of amyloid. Interestingly, the fibril-forming AgsA is able to suppress the dithiothreitol-induced aggregation of insulin efficiently within a certain range of temperature. During this process, AgsA fibrils disappear and spherical complexes form between AgsA and insulin molecules. These data suggest that AgsA fibrils may represent a distinctive type of structural and functional form of sHsp from spherical oligomers. Our study provides new insights into sHsp structures and chaperone functions. Structured summary of protein interactions: AgsA and AgsA bind by electron microscopy (View interaction). Insulin and Insulin bind by molecular sieving (View interaction). Insulin and Insulin bind by electron microscopy (View interaction). AgsA and AgsA bind by molecular sieving (View interaction). AgsA and AgsA bind by fluorescence technology (View interaction). AgsA and AgsA bind by circular dichroism (View interaction). Insulin and Insulin bind by fluorescence technology (View interaction). AgsA and Insulin bind by molecular sieving (View interaction). AgsA and AgsA bind by electron tomography (View interaction). (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000296575400008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); PubMed; 7; ARTICLE; 21; 3396-3402; 585
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/193813]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shi, Xiaodong,Wang, Zhao,Yan, Linxuan,et al. Small heat shock protein AgsA forms dynamic fibrils[J]. 欧洲生化学会联合会快报,2011.
APA	Shi, Xiaodong.,Wang, Zhao.,Yan, Linxuan.,Ezemaduka, Anastasia N..,Fan, Guizhen.,...&Chang, Zengyi.(2011).Small heat shock protein AgsA forms dynamic fibrils.欧洲生化学会联合会快报.
MLA	Shi, Xiaodong,et al."Small heat shock protein AgsA forms dynamic fibrils".欧洲生化学会联合会快报 (2011).