Directed Differentiation of Human Embryonic Stem Cells into Thymic   Epithelial Progenitor-like Cells Reconstitutes the Thymic   Microenvironment In Vivo

doi:10.1016/j.stem.2013.06.014

CORC > 北京大学 > 生命科学学院

	Directed Differentiation of Human Embryonic Stem Cells into Thymic Epithelial Progenitor-like Cells Reconstitutes the Thymic Microenvironment In Vivo
	Sun, Xiaoning ; Xu, Jun ; Lu, Hongxia ; Liu, Wang ; Miao, Zhenchuan ; Sui, Xin ; Liu, Haisong ; Su, Li ; Du, Weichao ; He, Qihua ; Chen, Fangyuan ; Shi, Yan ; Deng, Hongkui
刊名	cell stem cell
	2013
关键词	ENDODERM MICE ORGANOGENESIS GENERATION LUNG IDENTIFICATION INHIBITION MECHANISMS EXPRESSION TISSUE
DOI	10.1016/j.stem.2013.06.014
英文摘要	Thymus transplantation has great clinical potential for treating immunological disorders, but the shortage of transplant donors limits the progress of this therapy. Human embryonic stem cells (hESCs) are promising cell sources for generating thymic epithelial cells. Here, we report a stepwise protocol to direct the differentiation of hESCs into thymic epithelial progenitor-like cells (TEPLCs) by mimicking thymus organogenesis with sequential regulation of Activin, retinoic acid, BMP, and WNT signals. The hESC-derived TEPLCs expressed the key thymic marker gene FOXN1 and could further develop in vivo into thymic epithelium expressing the functional thymic markers MHC II and AIRE upon transplantation. Moreover, the TEPLC-derived thymic epithelium could supportmouse thymopoiesis in T-cell-deficient mice and promote human T cell generation in NOD/SCID mice engrafted with human hematopoietic stem cells (hHSCs). These findings could facilitate hESC-based replacement therapy and provide a valuable in vitro platform for studying human thymus organogenesis and regeneration.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000329570600015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell & Tissue Engineering; Cell Biology; SCI(E); PubMed; 28; ARTICLE; 2; 230-236; 13
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190417]
专题	生命科学学院
推荐引用方式 GB/T 7714	Sun, Xiaoning,Xu, Jun,Lu, Hongxia,et al. Directed Differentiation of Human Embryonic Stem Cells into Thymic Epithelial Progenitor-like Cells Reconstitutes the Thymic Microenvironment In Vivo[J]. cell stem cell,2013.
APA	Sun, Xiaoning.,Xu, Jun.,Lu, Hongxia.,Liu, Wang.,Miao, Zhenchuan.,...&Deng, Hongkui.(2013).Directed Differentiation of Human Embryonic Stem Cells into Thymic Epithelial Progenitor-like Cells Reconstitutes the Thymic Microenvironment In Vivo.cell stem cell.
MLA	Sun, Xiaoning,et al."Directed Differentiation of Human Embryonic Stem Cells into Thymic Epithelial Progenitor-like Cells Reconstitutes the Thymic Microenvironment In Vivo".cell stem cell (2013).