Genome-wide mapping of cellular protein-RNA interactions enabled by chemical crosslinking

doi:10.1016/j.gpb.2014.03.001

CORC > 北京大学 > 生命科学学院

	Genome-wide mapping of cellular protein-RNA interactions enabled by chemical crosslinking
	Li Xiaoyu ; Song Jinghui ; Yi Chengqi
刊名	genomics proteomics bioinformatics
	2014
关键词	Aza-IP,Crosslinking,High-throughput sequencing,Protein?RNA interactions,RNA-binding proteins,miCLIP
DOI	10.1016/j.gpb.2014.03.001
英文摘要	RNA-protein interactions influence many biological processes. Identifying the binding sites of RNA-binding proteins (RBPs) remains one of the most fundamental and important challenges to the studies of such interactions. Capturing RNA and RBPs via chemical crosslinking allows stringent purification procedures that significantly remove the non-specific RNA and protein interactions. Two major types of chemical crosslinking strategies have been developed to date, i.e., UV-enabled crosslinking and enzymatic mechanism-based covalent capture. In this review, we compare such strategies and their current applications, with an emphasis on the technologies themselves rather than the biology that has been revealed. We hope such methods could benefit broader audience and also urge for the development of new methods to study RNA-RBP interactions.Copyright ? 2014. Production and hosting by Elsevier Ltd.; PubMed; 中国科学引文数据库(CSCD); 0; 2; 72-8; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189498]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li Xiaoyu,Song Jinghui,Yi Chengqi. Genome-wide mapping of cellular protein-RNA interactions enabled by chemical crosslinking[J]. genomics proteomics bioinformatics,2014.
APA	Li Xiaoyu,Song Jinghui,&Yi Chengqi.(2014).Genome-wide mapping of cellular protein-RNA interactions enabled by chemical crosslinking.genomics proteomics bioinformatics.
MLA	Li Xiaoyu,et al."Genome-wide mapping of cellular protein-RNA interactions enabled by chemical crosslinking".genomics proteomics bioinformatics (2014).