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	Mouse SCNT ESCs Have Lower Somatic Mutation Load Than Syngeneic iPSCs
	Li, Zhe ; Lu, Hongxia ; Yang, Weifeng ; Yong, Jun ; Zhang, Zhen-ning ; Zhang, Kun ; Deng, Hongkui ; Xu, Yang
	2014
关键词	PLURIPOTENT STEM-CELLS NUCLEAR TRANSFER CODING MUTATIONS
DOI	10.1016/j.stemcr.2014.02.005
英文摘要	Ectopic expression of reprogramming factors has been widely adopted to reprogram somatic nucleus into a pluripotent state (induced pluripotent stem cells [iPSCs]). However, genetic aberrations such as somatic gene mutation in the resulting iPSCs have raised concerns regarding their clinical utility. To test whether the increased somatic mutations are primarily the by-products of current reprogramming methods, we reprogrammed embryonic fibroblasts of inbred C57BL/6 mice into either iPSCs (8 lines, 4 previously published) or embryonic stem cells (ESCs) with somatic cell nuclear transfer (SCNT ESCs; 11 lines). Exome sequencing of these lines indicates a significantly lower mutation load in SCNT ESCs than iPSCs of syngeneic background. In addition, one SCNT-ESC line has no detectable exome mutation, and two pairs of SCNT-ESC lines only have shared preexisting mutations. In contrast, every iPSC line carries unique mutations. Our study highlights the need for improving reprogramming methods in more physiologically relevant conditions.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336648800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell & Tissue Engineering; Cell Biology; SCI(E); PubMed; 11; ARTICLE; kzhang@bioeng.ucsd.edu; hongkui_deng@pku.edu.cn; yangxu@ucsd.edu; 4; 399-405; 2
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189493]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Zhe,Lu, Hongxia,Yang, Weifeng,et al. Mouse SCNT ESCs Have Lower Somatic Mutation Load Than Syngeneic iPSCs[J],2014.
APA	Li, Zhe.,Lu, Hongxia.,Yang, Weifeng.,Yong, Jun.,Zhang, Zhen-ning.,...&Xu, Yang.(2014).Mouse SCNT ESCs Have Lower Somatic Mutation Load Than Syngeneic iPSCs..
MLA	Li, Zhe,et al."Mouse SCNT ESCs Have Lower Somatic Mutation Load Than Syngeneic iPSCs".(2014).

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