Design, Construction, and Characterization of a Set of Biosensors for   Aromatic Compounds

doi:10.1021/sb500023f

CORC > 北京大学 > 生命科学学院

	Design, Construction, and Characterization of a Set of Biosensors for Aromatic Compounds
	Xue, Haoran ; Shi, Hailing ; Yu, Zhou ; He, Shuaixin ; Liu, Shiyu ; Hou, Yuhang ; Pan, Xingjie ; Wang, Huan ; Zheng, Pu ; Cui, Can ; Viets, Helena ; Liang, Jing ; Zhang, Yihao ; Chen, Shuobing ; Zhang, Haoqian M. ; Ouyang, Qi
刊名	acs synthetic biology
	2014
关键词	TRANSCRIPTIONAL ACTIVATORS GENE-EXPRESSION NTRC FAMILY IDENTIFICATION NAPHTHALENE METABOLISM REGULATORS BACTERIA MUTANTS MEMBER
DOI	10.1021/sb500023f
英文摘要	Aromatic pollutants in the environments pose significant threat to human health due to their persistence and toxicity. Here, we report the design and comprehensive characterization of a set of aromatic biosensors constructed using green fluorescence protein as the reporter and aromatics-responsive transcriptional regulators, namely, NahR, XylS, HbpR, and DmpR, as the detectors. The genetic connections between the detectors and the reporter were carefully adjusted to achieve fold inductions far exceeding those reported in previous studies. For each biosensor, the functional characteristics including the dose-responses, dynamic range, and the detection spectrum of aromatic species were thoroughly measured. In particular, the interferences that nontypical inducers exert on each biosensor's response to its strongest inducer were evaluated. These well-characterized biosensors might serve as potent tools for environmental monitoring as well as quantitative gene regulation.; Biochemical Research Methods; SCI(E); PubMed; 1; EDITORIAL MATERIAL; robinchen@pku.edu.cn; myelinzhang@pku.edu.cn; 12; 1011-1014; 3
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188688]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xue, Haoran,Shi, Hailing,Yu, Zhou,et al. Design, Construction, and Characterization of a Set of Biosensors for Aromatic Compounds[J]. acs synthetic biology,2014.
APA	Xue, Haoran.,Shi, Hailing.,Yu, Zhou.,He, Shuaixin.,Liu, Shiyu.,...&Ouyang, Qi.(2014).Design, Construction, and Characterization of a Set of Biosensors for Aromatic Compounds.acs synthetic biology.
MLA	Xue, Haoran,et al."Design, Construction, and Characterization of a Set of Biosensors for Aromatic Compounds".acs synthetic biology (2014).