Cardioprotection of recombinant human MG53 protein in a porcine model of ischemia and reperfusion injury

doi:10.1016/j.yjmcc.2014.12.010

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	Cardioprotection of recombinant human MG53 protein in a porcine model of ischemia and reperfusion injury
	Liu Jianxun ; Zhu Hua ; Zheng Yongqiu ; Xu Zhaobin ; Li Lei ; Tan Tao ; Park Ki Ho ; Hou Jincai ; Zhang Cuixiang ; Li Dan ; Li Ran ; Liu Zhenguo ; Weisleder Noah ; Zhu Desheng ; Lin Peihui ; Ma Jianjie
刊名	journal of molecular and cellular cardiology
	2015
关键词	Angioplasty,Cell membrane repair,Myocardial infarction,Myocardial ischemia,TRIM72
DOI	10.1016/j.yjmcc.2014.12.010
英文摘要	Ischemic heart disease is a leading cause of death in human population and protection of myocardial infarction (MI) associated with ischemia-reperfusion (I/R) remains a challenge. MG53 is an essential component of the cell membrane repair machinery that protects injury to the myocardium. We investigated the therapeutic value of using the recombinant human MG53 (rhMG53) protein for treatment of MI. Using Langendorff perfusion of isolated mouse heart, we found that I/R caused injury to cardiomyocytes and release of endogenous MG53 into the extracellular solution. rhMG53 protein was applied to the perfusion solution concentrated at injury sites on cardiomyocytes to facilitate cardioprotection. With rodent models of I/R-induced MI, we established the in vivo dosing range for rhMG53 in cardioprotection. Using a porcine model of angioplasty-induced MI, the cardioprotective effect of rhMG53 was evaluated. Intravenous administration of rhMG53, either prior to or post-ischemia, reduced infarct size and troponin I release in the porcine model when examined at 24h post-reperfusion. Echocardiogram and histological analyses revealed that the protective effects of rhMG53 observed following acute MI led to long-term improvement in cardiac structure and function in the porcine model when examined at 4weeks post-operation. Our study supports the concept that rhMG53 could have potential therapeutic value for treatment of MI in human patients with ischemic heart diseases.Copyright ? 2014 Elsevier Ltd. All rights reserved.; SCI(E); PubMed; 0; ARTICLE; Liujx0324@sina.com; Jianjie.Ma@osumc.edu; 10-9; 80
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188672]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu Jianxun,Zhu Hua,Zheng Yongqiu,et al. Cardioprotection of recombinant human MG53 protein in a porcine model of ischemia and reperfusion injury[J]. journal of molecular and cellular cardiology,2015.
APA	Liu Jianxun.,Zhu Hua.,Zheng Yongqiu.,Xu Zhaobin.,Li Lei.,...&Ma Jianjie.(2015).Cardioprotection of recombinant human MG53 protein in a porcine model of ischemia and reperfusion injury.journal of molecular and cellular cardiology.
MLA	Liu Jianxun,et al."Cardioprotection of recombinant human MG53 protein in a porcine model of ischemia and reperfusion injury".journal of molecular and cellular cardiology (2015).