Single cell sequencing: technique, application, and future development

doi:10.1007/s11434-014-0634-6

CORC > 北京大学 > 生命科学学院

	Single cell sequencing: technique, application, and future development
	Xue, Ruidong ; Li, Ruoyan ; Bai, Fan
刊名	science bulletin
	2015
关键词	Single cell isolation Genetic heterogeneity Single cell genome sequencing Single cell transcriptome sequencing Single cell methylome sequencing CIRCULATING TUMOR-CELLS EMBRYONIC STEM-CELLS MULTIPLE DISPLACEMENT AMPLIFICATION COPY NUMBER VARIATION MESSENGER-RNA-SEQ GENE-EXPRESSION MAMMALIAN DEVELOPMENT DNA SPERM SCALE
DOI	10.1007/s11434-014-0634-6
英文摘要	The progression of next generation sequencing is continuously changing the landscape of genomic, transcriptomic, and epigenomic studies. Particularly, advances in single cell manipulation and amplification techniques bring sequencing technology to the single-cell level. Single cell genome sequencing allows us to study tumor evolution, gamete genesis, somatic mosaicism at genome-wide level; single cell transcriptome sequencing unveils the dynamic gene expression during early embryonic development, differentiation and reprogramming; single cell methylome sequencing is just taking off and shows great potential in cancer and stem cell studies. Lots of attempts are still being made in other dimensions of sequencing. The increasing need for single cell sequencing requires the future techniques with the following features: (1) high accuracy and fidelity; (2) able to perform multiple omics analyses in one cell; (3) high degree of automation and standardized pipeline. These progresses and improvements will lower the barrier for single cell sequencing to enter ordinary laboratories. The wide application of single cell sequencing techniques will substantially change biomedical research in future.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000347924400005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 中国科学引文数据库(CSCD); 5; fbai@pku.edu.cn; 1; 33-42; 60
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162653]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xue, Ruidong,Li, Ruoyan,Bai, Fan. Single cell sequencing: technique, application, and future development[J]. science bulletin,2015.
APA	Xue, Ruidong,Li, Ruoyan,&Bai, Fan.(2015).Single cell sequencing: technique, application, and future development.science bulletin.
MLA	Xue, Ruidong,et al."Single cell sequencing: technique, application, and future development".science bulletin (2015).