The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication   Forks from Reversing

doi:10.1016/j.cell.2012.04.030

CORC > 北京大学 > 生命科学学院

	The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing
	Hu, Jiazhi ; Sun, Lei ; Shen, Fenfen ; Chen, Yufei ; Hua, Yu ; Liu, Yang ; Zhang, Mian ; Hu, Yiren ; Wang, Qingsong ; Xu, Wei ; Sun, Fei ; Ji, Jianguo ; Murray, Johanne M. ; Carr, Antony M. ; Kong, Daochun
刊名	cell
	2012
关键词	ESSENTIAL IN-VIVO SACCHAROMYCES-CEREVISIAE CHROMOSOMAL REARRANGEMENTS ENDONUCLEASE ACTIVITY DAMAGED DNA HOMOLOG YEAST KINASE EXO1 HELICASE/NUCLEASE
DOI	10.1016/j.cell.2012.04.030
英文摘要	When replication forks stall at damaged bases or upon nucleotide depletion, the intra-S phase checkpoint ensures they are stabilized and can restart. In intra-S checkpoint-deficient budding yeast, stalling forks collapse, and similar to 10% form pathogenic chicken foot structures, contributing to incomplete replication and cell death (Lopes et al., 2001; Sogo et al., 2002; Tercero and Diffley, 2001). Using fission yeast, we report that the Cds1(Chk2) effector kinase targets Dna2 on S220 to regulate, both in vivo and in vitro, Dna2 association with stalled replication forks in chromatin. We demonstrate that Dna2-S220 phosphorylation and the nuclease activity of Dna2 are required to prevent fork reversal. Consistent with this, Dna2 can efficiently cleave obligate precursors of fork regression-regressed leading or lagging strands-on model replication forks. We propose that Dna2 cleavage of regressed nascent strands prevents fork reversal and thus stabilizes stalled forks to maintain genome stability during replication stress.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305119600008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Cell Biology; SCI(E); PubMed; 63; ARTICLE; 6; 1221-1232; 149
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162509]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hu, Jiazhi,Sun, Lei,Shen, Fenfen,et al. The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing[J]. cell,2012.
APA	Hu, Jiazhi.,Sun, Lei.,Shen, Fenfen.,Chen, Yufei.,Hua, Yu.,...&Kong, Daochun.(2012).The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing.cell.
MLA	Hu, Jiazhi,et al."The Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing".cell (2012).