Reverse Genetic Approaches in Zebrafish

doi:10.1016/j.jgg.2012.07.004

CORC > 北京大学 > 生命科学学院

	Reverse Genetic Approaches in Zebrafish
	Huang, Peng ; Zhu, Zuoyan ; Lin, Shuo ; Zhang, Bo
刊名	遗传学报
	2012
关键词	Zebrafish Reverse genetics Morpholino TILLING Retrovirus ZFN TALEN Gene targeting ZINC-FINGER NUCLEASES MORPHOLINO ANTISENSE OLIGOMERS DNA-BINDING SPECIFICITY TAL EFFECTOR NUCLEASES MEDIATED ENHANCER TRAP DOUBLE-STRAND BREAKS IN-VIVO INSERTIONAL MUTAGENESIS VERTEBRATE DEVELOPMENT EMBRYO MICROINJECTION
DOI	10.1016/j.jgg.2012.07.004
英文摘要	Zebrafish (Danio rerio) is a well-established vertebrate animal model. A comprehensive collection of reverse genetics tools has been developed for studying gene function in this useful organism. Morpholino is the most widely used reagent to knock down target gene expression post-transcriptionally. For a long time, targeted genome modification has been heavily relied on large-scale traditional forward genetic screens, such as ENU (N-ethyl-N-nitrosourea) mutagenesis derived TILLING (Targeting Induced Local Lesions IN Genomes) strategy and pseudo-typed retrovirus mediated insertional mutagenesis. Recently, engineered endonucleases, including ZFNs (zinc finger nucleases) and TALENs (transcription activator-like effector nucleases), provide new and efficient strategies to directly generate site-specific indel mutations by inducing double strand breaks in target genes. Here we summarize the major reverse genetic approaches for loss-of-function studies used and emerging in zebrafish, including strategies based on genome-wide mutagenesis and methods for site-specific gene targeting. Future directions and expectations will also be discussed.; Biochemistry & Molecular Biology; Genetics & Heredity; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 30; 9; 421-433; 39
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162506]
专题	生命科学学院
推荐引用方式 GB/T 7714	Huang, Peng,Zhu, Zuoyan,Lin, Shuo,et al. Reverse Genetic Approaches in Zebrafish[J]. 遗传学报,2012.
APA	Huang, Peng,Zhu, Zuoyan,Lin, Shuo,&Zhang, Bo.(2012).Reverse Genetic Approaches in Zebrafish.遗传学报.
MLA	Huang, Peng,et al."Reverse Genetic Approaches in Zebrafish".遗传学报 (2012).