Quantitative proteomics and transcriptomics reveal key metabolic   processes associated with cotton fiber initiation

doi:10.1016/j.jprot.2014.10.022

CORC > 北京大学 > 生命科学学院

	Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation
	Wang, Xu-Chu ; Li, Qin ; Jin, Xiang ; Xiao, Guang-Hui ; Liu, Gao-Jun ; Liu, Nin-Jing ; Qin, Yong-Mei
刊名	journal of proteomics
	2015
关键词	Cotton fiber initiation Gossypium hirsutum Hydroxyacyl-CoA dehydratase iTRAQ RNA-sequencing Very long-chain fatty acid GOSSYPIUM-HIRSUTUM L. FATTY-ACID ELONGATION CELL ELONGATION ARABIDOPSIS-THALIANA TRICHOME DEVELOPMENT GENE-EXPRESSION DIFFERENTIATION ETHYLENE BIOSYNTHESIS PASTICCINO2
DOI	10.1016/j.jprot.2014.10.022
英文摘要	An iTRAQ-based proteomics of ovules from the upland cotton species Gossypiurn hirsutum and its fuzzless-lintless mutant was performed, and finally 2729 proteins that preferentially accumulated at anthesis in wild-type ovules were identified. We confirmed that the gene expression levels of 2005 among these proteins also increased by performing an RNA sequencing transcriptomics. Expression of proteins involved in carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, and lipid metabolism was significantly enhanced in wild-type ovules. Quantitative real-time PCR verified the increased expression of 26 genes involved in these processes. Cotton 3-hydroxyacyl-CoA dehydratase (GhPAS2) catalyzing the third reaction of very long-chain fatty acid (VLCFA) biosynthesis, accumulated at anthesis in wild-type ovules. Heterogeneous expression of GhPAS2 restored viability to the Saccharomyces cereuisiae haploid psh1-deletion strain deficient in PAS2 activity. Application of VLCFA biosynthesis inhibitor acetochlor (2-chloro-N-[ethoxymethyl]-N-[2-ethyl-6-methyl-phenl]-acetamide; ACE) and gibberellic acid to the unfertilized cotton ovules significantly suppressed fiber cell protrusion. In this study, the profiling of gene expression at both transcriptome and proteome levels provides new insights into cotton fiber cell initiation. Biological significance Cotton fiber initiation determines the ultimate number of fibers per ovule, thereby determining fiber yield. In total, 2729 proteins were preferentially accumulated in wild-type ovules at anthesis. The most up-regulated proteins were assigned to carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, and lipid metabolism. In consistence with these findings, we characterized GHPAS2 gene coding for the enzyme that catalyzes VLCFA production. VLCFA biosynthesis inhibitor, acetochlor, was shown to significantly suppress fiber initiation. This study provides a genome-scale transcriptomic and proteomic characterization of fiber initial cells, laying a solid basis for further investigation of the molecular processes governing fiber cell development (C) 2014 Elsevier B.V. All rights reserved.; Biochemical Research Methods; SCI(E); PubMed; 0; ARTICLE; qinym@pku.edu.cn; 16-27; 114
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162425]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Xu-Chu,Li, Qin,Jin, Xiang,et al. Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation[J]. journal of proteomics,2015.
APA	Wang, Xu-Chu.,Li, Qin.,Jin, Xiang.,Xiao, Guang-Hui.,Liu, Gao-Jun.,...&Qin, Yong-Mei.(2015).Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation.journal of proteomics.
MLA	Wang, Xu-Chu,et al."Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation".journal of proteomics (2015).