Novel mechanism of ethaselen in poorly differentiated colorectal RKO   cell growth inhibition: Simultaneous regulation of TrxR transcription,   expression and enzyme activity

doi:10.1016/j.diff.2010.09.180

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	Novel mechanism of ethaselen in poorly differentiated colorectal RKO cell growth inhibition: Simultaneous regulation of TrxR transcription, expression and enzyme activity
	Yin, Hanwei ; Li, Jing ; Xiong, Kun ; Wang, Lihui ; Wang, Tianyu ; Tan, Qiang ; Fu, Jianing ; Ren, Xiaoyuan ; Zeng, Huihui
刊名	differentiation
	2011
关键词	Thioredoxin reductase Ethaselen RKO cell LoVo cell Poorly differentiated Wnt/beta-catenin THIOREDOXIN REDUCTASE INHIBITOR CANCER STEM-CELLS BETA-CATENIN PROGENITOR CELLS TGF-BETA IN-VITRO APOPTOSIS PROTEIN GENE LINES
DOI	10.1016/j.diff.2010.09.180
英文摘要	Thioredoxin reductase (TrxR) is a ubiquitous intracellular redox enzyme that regulates tumor growth and proliferation in various cancer cells. Ethaselen (1,2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane), a novel anticancer agent, is designed to target mammalian TrxR1 with the aims of cancer growth inhibition and TrxR inactivation. In this study, we demonstrated that ethaselen significantly inhibits cell growth in the poorly differentiated colorectal RKO cell line, and simultaneously downregulates mammalian TrxR1 mRNA transcript levels, protein expression and enzyme activity, which differs from its actions in moderately differentiated colorectal LoVo cells. Ethaselen's significant abatement of the Wnt/beta-catenin cell differentiation-related signaling pathway was also observed in RKO cells; this apparently leads to its strong inhibitory effect on cell growth and TrxR1 activity in this cell line. These results suggest that ethaselen has a novel mechanism affecting cell growth in the poorly differentiated RKO colorectal cell line. (c) 2010 International Society of Differentiation. Published by Elsevier Ltd. All rights reserved.; Cell Biology; Developmental Biology; SCI(E); PubMed; 3; ARTICLE; 1; 49-56; 81
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/156698]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yin, Hanwei,Li, Jing,Xiong, Kun,et al. Novel mechanism of ethaselen in poorly differentiated colorectal RKO cell growth inhibition: Simultaneous regulation of TrxR transcription, expression and enzyme activity[J]. differentiation,2011.
APA	Yin, Hanwei.,Li, Jing.,Xiong, Kun.,Wang, Lihui.,Wang, Tianyu.,...&Zeng, Huihui.(2011).Novel mechanism of ethaselen in poorly differentiated colorectal RKO cell growth inhibition: Simultaneous regulation of TrxR transcription, expression and enzyme activity.differentiation.
MLA	Yin, Hanwei,et al."Novel mechanism of ethaselen in poorly differentiated colorectal RKO cell growth inhibition: Simultaneous regulation of TrxR transcription, expression and enzyme activity".differentiation (2011).