绿豆Ty1/Copia类反转录转座子逆转录酶序列的克隆和分析

doi:10.3321/j.issn:1672-9072.2004.05.011

CORC > 北京大学 > 生命科学学院

	绿豆Ty1/Copia类反转录转座子逆转录酶序列的克隆和分析; Isolation and Characterization of Ty1/Copia-like Reverse Transcriptase Sequences from Mung Bean
	肖卫民 ; 坂本亘 ; 苏都莫日根
刊名	植物学报
	2004
关键词	绿豆反转录转座子逆转录酶 DOP-PCR copia mung bean DOP-PCR copia retrotransposon reverse transcriptase
DOI	10.3321/j.issn:1672-9072.2004.05.011
英文摘要	利用Ty1/copia类反转录转座子的保守位点设计简并引物,从绿豆(Vigna radiata(L.)Wilczek)基因组中扩增得到了反转录转座子的逆转录酶序列.对扩增得到的约270bp的片段进行分离和克隆,并随机挑选了40个克隆进行测序,结果得到了36个单独的核酸序列,其中18个含有移码突变或终止子.根据序列比对,这些克隆可分为9组以及单个的9种.这40个克隆中,核酸序列相似性从8%到100%,显示出其核酸序列的高度异质性.将这些克隆的核酸序列与来自其他种植物的相应序列进行谱系分析,发现有些克隆与来自其他种植物的相应序列的亲缘关系比这些克隆之间更为接近.斑点杂交显示Ty1/copia反转录转座子约占绿豆基因组的9.3%.; Ty1/copia-like sequences were amplified from mung bean ( Vigna radiata (L.) Wilczek) genomic DNA, by PCR with degenerate oligonucleotide primers corresponding to highly conserved domains in the Ty1/copia-like retrotransposons. PCR fragments of roughly 270 bp were isolated and cloned, and forty clones were sequenced. Thirty-six of the forty clones had unique nucleotide sequences, and eighteen clones had a frameshift, a stop codon, or both. Alignment of the nucleotide sequences indicated that these clones, denoted Tvr, fell into nine subgroups and nine ungrouped sequences. The nucleotide sequence similarity between these elements ranged from 8% to 100%, which indicates high level of sequence heterogeneity among these clones. A phylogenetic analysis comparing these clones with corresponding sequences from other plant species showed that some of the Tvr clones are more closely related to Ty1/copia-like retrotransposons from other species than to other Tvr clones. Dot blot analysis revealed that Ty1/copia-like retrotransposons comprise about 9.3% of the mung bean genome.; 国家重点基础研究发展计划(973计划); 国家自然科学基金; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 3; 5; 582-587; 46
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/46970]
专题	生命科学学院
推荐引用方式 GB/T 7714	肖卫民,坂本亘,苏都莫日根. 绿豆Ty1/Copia类反转录转座子逆转录酶序列的克隆和分析, Isolation and Characterization of Ty1/Copia-like Reverse Transcriptase Sequences from Mung Bean[J]. 植物学报,2004.
APA	肖卫民,坂本亘,&苏都莫日根.(2004).绿豆Ty1/Copia类反转录转座子逆转录酶序列的克隆和分析.植物学报.
MLA	肖卫民,et al."绿豆Ty1/Copia类反转录转座子逆转录酶序列的克隆和分析".植物学报 (2004).