Tautomerization-dependent recognition and excision of oxidation damage   in base-excision DNA repair

doi:10.1073/pnas.1604591113

CORC > 北京大学 > 化学与分子工程学院

	Tautomerization-dependent recognition and excision of oxidation damage in base-excision DNA repair
	Zhu, Chenxu ; Lu, Lining ; Zhang, Jun ; Yue, Zongwei ; Song, Jinghui ; Zong, Shuai ; Liu, Menghao ; Stovicek, Olivia ; Gao, Yi Qin ; Yi, Chengqi
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2016
关键词	base-excision repair substrate recognition enzyme catalysis glycosylase QM/MM COLI ENDONUCLEASE-VIII THYMINE GLYCOL LESIONS C-TERMINAL DOMAIN NEIL1 DNA GLYCOSYLASE NEIL1 STEREOSELECTIVE EXCISION REPLICATION PROTEINS STRUCTURAL INSIGHTS HYDANTOIN LESIONS CRYSTAL-STRUCTURE
DOI	10.1073/pnas.1604591113
英文摘要	NEIL1 (Nei-like 1) is a DNA repair glycosylase guarding the mammalian genome against oxidized DNA bases. As the first enzymes in the base-excision repair pathway, glycosylases must recognize the cognate substrates and catalyze their excision. Here we present crystal structures of human NEIL1 bound to a range of duplex DNA. Together with computational and biochemical analyses, our results suggest that NEIL1 promotes tautomerization of thymine glycol (Tg)-a preferred substrate-for optimal binding in its active site. Moreover, this tautomerization event also facilitates NEIL1-catalyzed Tg excision. To our knowledge, the present example represents the first documented case of enzyme-promoted tautomerization for efficient substrate recognition and catalysis in an enzyme-catalyzed reaction.; National Basic Research Foundation of China [2014CB964900]; National Natural Science Foundation of China [31270838, 21522201, 21573006]; SCI(E); PubMed; ARTICLE; gaoyq@pku.edu.cn; chengqi.yi@pku.edu.cn; 28; 7792-7797; 113
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/491905]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Zhu, Chenxu,Lu, Lining,Zhang, Jun,et al. Tautomerization-dependent recognition and excision of oxidation damage in base-excision DNA repair[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016.
APA	Zhu, Chenxu.,Lu, Lining.,Zhang, Jun.,Yue, Zongwei.,Song, Jinghui.,...&Yi, Chengqi.(2016).Tautomerization-dependent recognition and excision of oxidation damage in base-excision DNA repair.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
MLA	Zhu, Chenxu,et al."Tautomerization-dependent recognition and excision of oxidation damage in base-excision DNA repair".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016).