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	Study of G-quadruplexes in the STAT3 gene using electrospray ionization mass spectrometry
	Lin, Sen ; Long, Haitao ; Zhou, Jiang ; Yuan, Gu
	2016
关键词	CIRCULAR-DICHROISM PROMOTER REGION SMALL-MOLECULE TELOMERIC DNA RECOGNITION TRANSCRIPTION THERAPY BINDING HEART
英文摘要	RATIONALE: As a key signal transducer and transcription activator, STAT3 plays a very important role in many cell processes. We found that there were many G-rich sequences existing in the STAT3 gene including its promoter, intron, exon and 3'-flanking regions. These G-rich tracts can form G-quadruplexes under near physiological conditions. In this research, we systemically studied the G-quadruplexes in the STAT3 gene at a whole gene scale for the first time. METHODS: In this research, the formation of G-quadruplexes in the STAT3 gene was probed by electrospray ionization mass spectrometry (ESI-MS) and circular dichroism (CD). Their structures were constructed and refined by a molecular modeling method. We also used ESI-MS to study the recognition of the G-quadruplexes in the promoter of the STAT3 gene by flexible molecules which do not have a planar core like the other common quadruplex ligands. RESULTS: The results based on ESI-MS suggested that the G-quadruplexes in the promoter of the STAT3 gene formed and were further recognized by some small molecules. CONCLUSION: Our research proved that the G-rich sequences in the STAT3 gene could form G-quadruplexes under near physiological conditions. This provides a promising target to study the regulation of cell signal transduction in vivo and drug design that aims to target STAT3 G-quadruplexes. Copyright (C) 2016 John Wiley & Sons, Ltd.; SCI(E); CPCI-S(ISTP); zhoujiang@pku.edu.cn; guyuan@pku.edu.cn; ,SI; 173-178; 30
语种	英语
出处	2nd National Conference on Mass Spectrometry Analysis of China
DOI标识	10.1002/rcm.7640
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/491669]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Lin, Sen,Long, Haitao,Zhou, Jiang,et al. Study of G-quadruplexes in the STAT3 gene using electrospray ionization mass spectrometry. 2016-01-01.

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