Exploration of a multi-target ligand, dehydroevodiamine, for the   recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI-MS

doi:10.1016/j.ijms.2017.01.006

CORC > 北京大学 > 化学与分子工程学院

	Exploration of a multi-target ligand, dehydroevodiamine, for the recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI-MS
	Li, Fangyuan ; Zhou, Jiang ; Xu, Ming ; Yuan, Gu
刊名	INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
	2017
关键词	c-Myb G-quadruplex ESI-MS Dehydroevodiamine MASS-SPECTROMETRY BINDING-AFFINITY P1 PROMOTER DNA TRANSCRIPTION DERIVATIVES SELECTIVITY ONCOGENE UPSTREAM REGION
DOI	10.1016/j.ijms.2017.01.006
英文摘要	In this research, ESI mass spectrometry was used to probe the formation of G-quadruplexes from three G-rich sequences (S1-S3) in the upstream region of the transcription start site in human c-Myb protooncogene. A ligand, dehydroevodiamine, was found for its high binding affinities towards the c-Myb G-quadruplexes. In addition, dehydroevodiamine bound towards the Q1-Q3 with high selectivity over long-chain duplex DNA. High-resolution ESI-MS was utilized to investigate the binding competition of dehydroevodiamine in the mixture solution of Q1-Q3 G-quadruplexes, and it appeared to have the binding affinity in the following order: Q3 approximate to Q1 > Q2, which is consistent with the results in the single G-quadruplex solutions. The properties of dehydroevodiamine gave its potential in the future studies of c-Myb expression regulation. (C) 2017 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21372021, 21572016]; 973 Program [2012CB720600, 2012CB720601]; SCI(E); ARTICLE; 39-44; 414
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474781]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Fangyuan,Zhou, Jiang,Xu, Ming,et al. Exploration of a multi-target ligand, dehydroevodiamine, for the recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI-MS[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2017.
APA	Li, Fangyuan,Zhou, Jiang,Xu, Ming,&Yuan, Gu.(2017).Exploration of a multi-target ligand, dehydroevodiamine, for the recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI-MS.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY.
MLA	Li, Fangyuan,et al."Exploration of a multi-target ligand, dehydroevodiamine, for the recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI-MS".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY (2017).