Quantitative analysis of Shigella flexneri protein expression under acid   stress

doi:10.1002/pmic.201600381

CORC > 北京大学 > 化学与分子工程学院

	Quantitative analysis of Shigella flexneri protein expression under acid stress
	Yu, Kaiwen ; Wang, Zhen ; Zhou, Fan ; Jiang, Jiezhang ; Liu, Yanhua ; Hu, Mo ; Liu, Xiaoyun
刊名	PROTEOMICS
	2017
关键词	Acid resistance Acid stress Protein expression Shigella flexneri ESCHERICHIA-COLI K-12 GENE-EXPRESSION RESISTANCE PH SURVIVAL TYPHIMURIUM MECHANISMS CATABOLISM BACTERIA
DOI	10.1002/pmic.201600381
英文摘要	As an important foodborne pathogen, Shigella flexneri can cause widespread enteric infection with bacteria as few as hundreds. This is, at least in part, attributed to its robust anti-acid strategies because passage through the highly acidic human digestive tract is a prerequisite for successful bacterial infection. Nevertheless, our understanding of these mechanisms and the impact of acid stress on Shigella protein expression still remains largely incomplete. Herein we conducted a proteomic survey of Shigella spp. under acid stress. Out of 1754 protein identifications, we found 131 altered proteins, most of which were down-regulated, including virulence factors and cell envelope proteins. Rather, many metabolic enzymes and pyrimidine/amino acid biosynthesis proteins were up-regulated. In addition to induction ofmany known anti-acid systems, we also found marked increase of 2-oxoglutarate dehydrogenase (SucAB), a metabolic enzyme in the tricarboxylic acid cycle. Importantly, overproduction of this enzyme significantly enhanced Shigella acid resistance and hence SucAB-mediated metabolic pathways may represent novel anti-acid strategies.; National Natural Science Foundation of China [21475005, 21622501]; Thousand Young Talents program from the Chinese government; SCI(E); ARTICLE; 8; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474214]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Yu, Kaiwen,Wang, Zhen,Zhou, Fan,et al. Quantitative analysis of Shigella flexneri protein expression under acid stress[J]. PROTEOMICS,2017.
APA	Yu, Kaiwen.,Wang, Zhen.,Zhou, Fan.,Jiang, Jiezhang.,Liu, Yanhua.,...&Liu, Xiaoyun.(2017).Quantitative analysis of Shigella flexneri protein expression under acid stress.PROTEOMICS.
MLA	Yu, Kaiwen,et al."Quantitative analysis of Shigella flexneri protein expression under acid stress".PROTEOMICS (2017).