Sap1 is a replication-initiation factor essential for the assembly of   pre-replicative complex in the fission yeast Schizosaccharomyces pombe

doi:10.1074/jbc.M116.767806

CORC > 北京大学 > 化学与分子工程学院

	Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe
	Guan, Ling ; He, Peng ; Yang, Fang ; Zhang, Yuan ; Hu, Yunfei ; Ding, Jienv ; Hua, Yu ; Zhang, Yi ; Ye, Qiong ; Hu, Jiazhi ; Wang, Tao ; Jin, Changwen ; Kong, Daochun
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2017
关键词	ORIGIN RECOGNITION COMPLEX DNA-REPLICATION ORC-BINDING PROTEIN SITES P65(CDC18) CONSISTS DYNAMICS MUTANTS DOMAINS
DOI	10.1074/jbc.M116.767806
英文摘要	A central step in the initiation of chromosomal DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) at late M and early G(1) phase of the cell cycles. Since 1973, four proteins or protein complexes, including cell division control protein 6 (Cdc6)/Cdc18, minichromosome maintenance protein complex, origin recognition complex (ORC), and Cdt1, are known components of the pre-RC. Previously, we reported that a non-ORC protein binds to the essential element Delta 9 of the Schizosaccharomyces pombe DNA-replication origin ARS3001. In this study, we identified that the non-ORC protein is Sap1. Like ORC, Sap1 binds to DNA origins during cell growth cycles. But unlike ORC, which binds to asymmetric AT-rich sequences through its nine AT-hook motifs, Sap1 preferentially binds to a DNA sequence of 5'-(A/T)(n)(C/G)(A/T)(9-10)(G/C)(A/T)(n)-3' (n >= 1). We also found that Sap1 and ORC physically interact. We further demonstrated that Sap1 is required for the assembly of the pre-RC because of its essential role in recruiting Cdc18 to DNA origins. Thus, we conclude that Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1.; National Natural Science Foundation of China [30770441, 31170739, 31230021, 31300600]; Ministry of Science and Technology of China [2006CB910300, 2010CB912201, 2013CB911000, 2013CB911500]; 985 Program; National Key Laboratory of Protein and Plant Gene Research; Peking-Tsinghua Center for Life Sciences; Shenzhen Science and technology Innovation (SZSTI) [JCYJ20160331115853521, GGJS20130329180714793]; National Key Research and Development Plan [2016YFA0501201]; SCI(E); ARTICLE; 15; 6056-6075; 292
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474000]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Guan, Ling,He, Peng,Yang, Fang,et al. Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017.
APA	Guan, Ling.,He, Peng.,Yang, Fang.,Zhang, Yuan.,Hu, Yunfei.,...&Kong, Daochun.(2017).Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe.JOURNAL OF BIOLOGICAL CHEMISTRY.
MLA	Guan, Ling,et al."Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe".JOURNAL OF BIOLOGICAL CHEMISTRY (2017).