A bioassay-guided fractionation system to identify endogenous small   molecules that activate plasma membrane H+-ATPase activity in   Arabidopsis

doi:10.1093/jxb/erx156

CORC > 北京大学 > 化学与分子工程学院

	A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis
	Han, Xiuli ; Yang, Yongqing ; Wu, Yujiao ; Liu, Xiaohui ; Lei, Xiaoguang ; Guo, Yan
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2017
关键词	Arabidopsis bioassay-guided fractionation plasma membrane H+-ATPase unsaturated fatty acids C-TERMINAL REGION FATTY-ACID 14-3-3 PROTEIN CELL EXPANSION PLANT-GROWTH SALT STRESS THALIANA LYSOPHOSPHATIDYLCHOLINE PHOSPHORYLATION DESATURASE
DOI	10.1093/jxb/erx156
英文摘要	Plasma membrane (PM) H+-ATPase is essential for plant growth and development. Various environmental stimuli regulate its activity, a process that involves many protein cofactors. However, whether endogenous small molecules play a role in this regulation remains unknown. Here, we describe a bio-guided isolation method to identify endogenous small molecules that regulate PM H+-ATPase activity. We obtained crude extracts from Arabidopsis seedlings with or without salt treatment and then purified them into fractions based on polarity and molecular mass by repeated column chromatography. By evaluating the effect of each fraction on PM H+-ATPase activity, we found that fractions containing the endogenous, free unsaturated fatty acids oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3) extracted from salt-treated seedlings stimulate PM H+-ATPase activity. These results were further confirmed by the addition of exogenous C18:1, C18:2, or C18:3 in the activity assay. The ssi2 mutant, with reduced levels of C18:1, C18:2, and C18:3, displayed reduced PM H+-ATPase activity. Furthermore, C18:1, C18:2, and C18:3 directly bound to the C-terminus of the PM H+-ATPase AHA2. Collectively, our results demonstrate that the binding of free unsaturated fatty acids to the C-terminus of PM H+-ATPase is required for its activation under salt stress. The bio-guided isolation model described in this study could enable the identification of new endogenous small molecules that modulate essential protein functions, as well as signal transduction, in plants.; National Natural Science Foundation of China [31430012, 31670260]; Foundation for Innovative Research Group of the National Natural Science Foundation of China [31421062]; National Genetically Modified Organisms Breeding Major Projects [2016ZX08009002]; SCI(E); ARTICLE; 11; 2951-2962; 68
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473376]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Han, Xiuli,Yang, Yongqing,Wu, Yujiao,et al. A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2017.
APA	Han, Xiuli,Yang, Yongqing,Wu, Yujiao,Liu, Xiaohui,Lei, Xiaoguang,&Guo, Yan.(2017).A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis.JOURNAL OF EXPERIMENTAL BOTANY.
MLA	Han, Xiuli,et al."A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis".JOURNAL OF EXPERIMENTAL BOTANY (2017).