NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells   with Hemicyanine Derivative-Assembled Upconversion Nanophosphors

doi:10.1021/acs.analchem.7b01324

CORC > 北京大学 > 化学与分子工程学院

	NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells with Hemicyanine Derivative-Assembled Upconversion Nanophosphors
	Li, Haixia ; Dong, Hao ; Yu, Mingming ; Liu, Chunxia ; Li, Zhanxian ; Wei, Liuhe ; Sun, Ling-Dong ; Zhang, Hongyan
刊名	ANALYTICAL CHEMISTRY
	2017
关键词	NEAR-INFRARED FLUORESCENCE INTRACELLULAR PH IN-VIVO ENERGY-TRANSFER NANOPARTICLES SENSOR NANOPROBES PROBES THERAPY CONSTRUCTION
DOI	10.1021/acs.analchem.7b01324
英文摘要	It is crucial for cell physiology to keep the homeostasis of pH, and it is highly demanded yet challenging to develop luminescence resonance energy transfer (LRET)-based near-infrared (NIR) ratiometric luminescent sensor for the detection of pH fluctuation with NIR excitation. As promising energy donors for LRET, upconversion nanoparticles (UCNPs) have been widely used to fabricate nanosensors, but the relatively low LRET efficiency limits their application in bioassay. To improve the LRET efficiency, core/shell/shell structured beta-NaGdF4@NaYF4:Yb,Tm@NaYF4 UCNPs were prepared and decorated with hemicyanine dyes as an LRET-based NIR ratiometric luminescent pH fluctuation-nanosensor for the first time. The as-developed nanosensor not only exhibits good antidisturbance ability, but it also can reversibly sense pH and linearly sense pH in a range of 6.0-9.0 and 6.8-9.0 from absorption and upconversion emission spectra, respectively. In addition, the nanosensor displays low dark toxicity under physiological temperature, indicating good biocompatibility. Furthermore, live cell imaging results revealed that the sensor can selectively monitor pH fluctuation via ratiometric upconversion luminescence behavior.; Key Laboratory of Photochemical Conversion and Optoelectronic Materials; Technical Institute of Physics and Chemistry; Chinese Academy of Sciences; NNSF of China [21601158, U1504203, 21590791, 21425101]; Zhengzhou University [32210431]; SCI(E); ARTICLE; 17; 8863-8869; 89
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470938]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Haixia,Dong, Hao,Yu, Mingming,et al. NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells with Hemicyanine Derivative-Assembled Upconversion Nanophosphors[J]. ANALYTICAL CHEMISTRY,2017.
APA	Li, Haixia.,Dong, Hao.,Yu, Mingming.,Liu, Chunxia.,Li, Zhanxian.,...&Zhang, Hongyan.(2017).NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells with Hemicyanine Derivative-Assembled Upconversion Nanophosphors.ANALYTICAL CHEMISTRY.
MLA	Li, Haixia,et al."NIR Ratiometric Luminescence Detection of pH Fluctuation in Living Cells with Hemicyanine Derivative-Assembled Upconversion Nanophosphors".ANALYTICAL CHEMISTRY (2017).