Precise Labeling and Tracking of Lipid Droplets in Adipocytes Using a   Luminescent ZnSalen Complex

doi:10.1002/asia.201701010

CORC > 北京大学 > 化学与分子工程学院

	Precise Labeling and Tracking of Lipid Droplets in Adipocytes Using a Luminescent ZnSalen Complex
	Tang, Juan ; Zhang, Yanfei ; Yin, Hao-Yan ; Xu, Guoheng ; Zhang, Jun-Long
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2017
关键词	cell imaging chemical biology fluorescence microscopy lipid droplets luminescent metal complex SEOUL-FLUOR 2-PHOTON MICROSCOPY METAL-COMPLEXES LIVE CELLS IN-VIVO FLUORESCENCE PROBE ACCUMULATION ENDOCYTOSIS AGGREGATION
DOI	10.1002/asia.201701010
英文摘要	Designing two-photon probes for precise labeling of lipid droplets (LDs) and monitoring LD dynamics in adipocytes is of great significance to understand LD homeostasis. We herein report that a luminescent metal complex LD-TPZn can specifically image LDs in adipose cells and tissue using one- or two-photon fluorescence microscopy. Importantly, LD-TPZn exhibited higher specificity to LDs than the commercial dyes Nile Red and Bodipy 493/503, probably due to different cellular uptake pathways, that is, clathrin-mediated endocytosis and non-selectively passive diffusion, respectively. More importantly, LD-TPZn can be applied as a two-photon LD probe to image adipose tissue, one of the most challengeable tissues for traditional one-photon fluorescence microscope imaging due to the strong light scattering. Most importantly, LD-TPZn can be used to monitor LD growth during adipogenesis of preadipocytes, which is highly desirable to unravel the relationship between LD homeostasis and metabolic diseases.; National Key Basic Research Support Foundation of China [2015CB856301]; National Scientific Foundation of China [21571007, 21271013, 21321001, 81570791]; SCI(E); ARTICLE; 19; 2533-2538; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470496]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Tang, Juan,Zhang, Yanfei,Yin, Hao-Yan,et al. Precise Labeling and Tracking of Lipid Droplets in Adipocytes Using a Luminescent ZnSalen Complex[J]. CHEMISTRY-AN ASIAN JOURNAL,2017.
APA	Tang, Juan,Zhang, Yanfei,Yin, Hao-Yan,Xu, Guoheng,&Zhang, Jun-Long.(2017).Precise Labeling and Tracking of Lipid Droplets in Adipocytes Using a Luminescent ZnSalen Complex.CHEMISTRY-AN ASIAN JOURNAL.
MLA	Tang, Juan,et al."Precise Labeling and Tracking of Lipid Droplets in Adipocytes Using a Luminescent ZnSalen Complex".CHEMISTRY-AN ASIAN JOURNAL (2017).