Scanning Single-Molecule Fluorescence Correlation Spectroscopy Enables   Kinetics Study of DNA Hairpin Folding with a Time Window from   Microseconds to Seconds

doi:10.1021/acs.jpclett.6b00720

CORC > 北京大学 > 化学与分子工程学院

	Scanning Single-Molecule Fluorescence Correlation Spectroscopy Enables Kinetics Study of DNA Hairpin Folding with a Time Window from Microseconds to Seconds
	Bi, Huimin ; Yin, Yandong ; Pan, Bailong ; Li, Geng ; Zhao, Xin Sheng
刊名	JOURNAL OF PHYSICAL CHEMISTRY LETTERS
	2016
关键词	CONFORMATIONAL FLUCTUATIONS ELECTRON-TRANSFER LIVE CELLS DYNAMICS FLOW OLIGONUCLEOTIDES GUANOSINE DIFFUSION PROTEINS BASE
DOI	10.1021/acs.jpclett.6b00720
英文摘要	Single-molecule fluorescence measurements have been widely used to explore kinetics and dynamics of biological systems. Among them, single-molecule imaging (SMI) is good at tracking processes slower than tens of milliseconds, whereas fluorescence correlation spectroscopy (FCS) is good at probing processes faster than submilliseconds. However, there is still shortage of simple yet effective single-molecule fluorescence method to cover the time-scale between submilliseconds and tens of milliseconds. To effectively bridge this millisecond gap, we developed a single-molecule fluorescence correlation spectroscopy (smFCS) method that works on surface-immobilized single molecules through surface scanning. We validated it by monitoring the classical DNA hairpin folding process. With a wide time window from microseconds to seconds, the experimental data are well fitted to the two-state folding model. All relevant molecular parameters, including the relative fluorescence brightness, equilibrium constant, and reaction rate constants, were uniquely determined.; NKBRSF [2012CB917304]; NSFC [21233002, 21521003]; SCI(E); ARTICLE; zhaoxs@pku.edu.cn; 10; 1865-1871; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/438166]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Bi, Huimin,Yin, Yandong,Pan, Bailong,et al. Scanning Single-Molecule Fluorescence Correlation Spectroscopy Enables Kinetics Study of DNA Hairpin Folding with a Time Window from Microseconds to Seconds[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2016.
APA	Bi, Huimin,Yin, Yandong,Pan, Bailong,Li, Geng,&Zhao, Xin Sheng.(2016).Scanning Single-Molecule Fluorescence Correlation Spectroscopy Enables Kinetics Study of DNA Hairpin Folding with a Time Window from Microseconds to Seconds.JOURNAL OF PHYSICAL CHEMISTRY LETTERS.
MLA	Bi, Huimin,et al."Scanning Single-Molecule Fluorescence Correlation Spectroscopy Enables Kinetics Study of DNA Hairpin Folding with a Time Window from Microseconds to Seconds".JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2016).