Direct Measurement of Single-Molecule DNA Hybridization Dynamics with   Single-Base Resolution

doi:10.1002/anie.201603038

CORC > 北京大学 > 化学与分子工程学院

	Direct Measurement of Single-Molecule DNA Hybridization Dynamics with Single-Base Resolution
	He, Gen ; Li, Jie ; Ci, Haina ; Qi, Chuanmin ; Guo, Xuefeng
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2016
关键词	DNA hybridization single-base resolution single-molecule devices FLUORESCENCE CORRELATION SPECTROSCOPY ELECTRICAL DETECTION SILICON NANOWIRES KINETICS HAIRPIN PLATFORM SENSORS
DOI	10.1002/anie.201603038
英文摘要	Herein, we report label-free detection of single-molecule DNA hybridization dynamics with single-base resolution. By using an electronic circuit based on point-decorated silicon nanowires as electrical probes, we directly record the folding/unfolding process of individual hairpin DNAs with sufficiently high signal-to-noise ratio and bandwidth. These measurements reveal two-level current oscillations with strong temperature dependence, enabling us to determine the thermodynamic and kinetic properties of hairpin DNA hybridization. More importantly, successive, stepwise increases and decreases in device conductance at low temperature on a microsecond timescale are successfully observed, indicating a base-by-base unfolding/folding process. The process demonstrates a kinetic zipper model for DNA hybridization/dehybridization at the single base-pair level. This measurement capability promises a label-free single-molecule approach to probe biomolecular interactions with fast dynamics.; NSFC [21225311, 91333102, 21373014, 21571021]; 973 Project [2012CB921404]; SCI(E); EI; PubMed; ARTICLE; qichuanmin@bnu.edu.cn; guoxf@pku.edu.cn; 31; 9036-9040; 55
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433428]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	He, Gen,Li, Jie,Ci, Haina,et al. Direct Measurement of Single-Molecule DNA Hybridization Dynamics with Single-Base Resolution[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016.
APA	He, Gen,Li, Jie,Ci, Haina,Qi, Chuanmin,&Guo, Xuefeng.(2016).Direct Measurement of Single-Molecule DNA Hybridization Dynamics with Single-Base Resolution.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
MLA	He, Gen,et al."Direct Measurement of Single-Molecule DNA Hybridization Dynamics with Single-Base Resolution".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2016).