Inhibition of nicotine-DNA adduct formation by polyphenolic compounds in vitro

CORC > 北京大学 > 化学与分子工程学院

	Inhibition of nicotine-DNA adduct formation by polyphenolic compounds in vitro
	CHENG Yan ; WANG Hai-Fang ; SUN Hong-Fang ; LI Hong-Li
刊名	Nuclear Science and Techniques
	2004
关键词	Nicotine DNA adducts Liquid scintillation counting Inhibition Polyphenols Biotransformation enzymes
英文摘要	Nicotine [3-(1-methyl-2-pyrrolidinyl)-pyridine], a major alkaloid in tobacco products, has proven to be a potential genotoxic compound. Some polyphenolic compounds can suppress the DNA adduction, and hence act as the potential inhibitors of carcinogenesis. In this study, the inhibitory effects of three polyphenolic compounds, curcumin (diferuloylmethane), resveratrol (trans-3, 5, 4-trihydroxystilbene) and tea polyphenols, on the nicotine-DNA adduction have been investigated in vitro using radiolabelled nicotine and liquid scintillation counting (LSC) technique. Also, the inhibition mechanism of these chemopreventive agents in regard to the activity of the biotransformation enzymes, including cytochrome P450 (CYP450), cytochrome b5 (CYb5) and glutathione S-transferase (GST), has been studied. The results demonstrated that these three polyphenols induced marked dose-dependent decrease in nicotine-DNA adducts as compared with the controls. The elimination rate of adducts reached above 46% at the highest dose for all the three agents with 51.6% for resveratrol. Correspondingly, three polyphenols all suppressed CYP450 and CYb5, whereas curcumin and resveratrol induced GST. We may arrive at a point that the three polyphenols are beneficial to prevent the nicotine adduct formation, and thus may be used to block the potential carcinogenesis induced by nicotine.; Supported by the National Natural Science Foundation of China; 中国科学引文数据库(CSCD); 4; 219-222; 15
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/426471]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	CHENG Yan,WANG Hai-Fang,SUN Hong-Fang,et al. Inhibition of nicotine-DNA adduct formation by polyphenolic compounds in vitro[J]. Nuclear Science and Techniques,2004.
APA	CHENG Yan,WANG Hai-Fang,SUN Hong-Fang,&LI Hong-Li.(2004).Inhibition of nicotine-DNA adduct formation by polyphenolic compounds in vitro.Nuclear Science and Techniques.
MLA	CHENG Yan,et al."Inhibition of nicotine-DNA adduct formation by polyphenolic compounds in vitro".Nuclear Science and Techniques (2004).