The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ   Cells

doi:10.1016/j.cell.2015.05.015

CORC > 北京大学 > 化学与分子工程学院

	The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells
	Guo, Fan ; Yan, Liying ; Guo, Hongshan ; Li, Lin ; Hu, Boqiang ; Zhao, Yangyu ; Yong, Jun ; Hu, Yuqiong ; Wang, Xiaoye ; Wei, Yuan ; Wang, Wei ; Li, Rong ; Yan, Jie ; Zhi, Xu ; Zhang, Yan ; Jin, Hongyan ; Zhang, Wenxin ; Hou, Yu ; Zhu, Ping ; Li, Jingyun ; Zhang, Ling ; Liu, Sirui ; Ren, Yixin ; Zhu, Xiaohui ; Wen, Lu ; Gao, Yi Qin ; Tang, Fuchou ; Qiao, Jie
刊名	CELL
	2015
关键词	EMBRYONIC STEM-CELLS SINGLE-CELL GENE-EXPRESSION DEMETHYLATION GENOME DYNAMICS MICE SPECIFICATION PATHWAY ERASURE
DOI	10.1016/j.cell.2015.05.015
英文摘要	Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental-stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10 to 11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation, with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.; National Natural Science of China [31230047, 31322037, 31271543]; National Basic Research Program of China [2011CB944504, 2014CB943200, 2012CB966704, 2011CB966303]; Beijing Municipal Science and Technology Commission [Z131100005213006]; Peking University for translation research; National Natural Science Foundation of China [31440063]; Peking-Tsinghua Center for Life Sciences; Center for Molecular and Translational Medicine; SCI(E); PubMed; ARTICLE; tangfuchou@pku.edu.cn; jie.qiao@263.net; 6; 1437-1452; 161
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/419419]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Guo, Fan,Yan, Liying,Guo, Hongshan,et al. The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells[J]. CELL,2015.
APA	Guo, Fan.,Yan, Liying.,Guo, Hongshan.,Li, Lin.,Hu, Boqiang.,...&Qiao, Jie.(2015).The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells.CELL.
MLA	Guo, Fan,et al."The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells".CELL (2015).