Second Generation TQ-Ligation for Cell Organelle Imaging

doi:10.1021/acschembio.5b00193

CORC > 北京大学 > 化学与分子工程学院

	Second Generation TQ-Ligation for Cell Organelle Imaging
	Zhang, Xiaoyun ; Dong, Ting ; Li, Qiang ; Liu, Xiaohui ; Li, Lin ; Chen, She ; Lei, Xiaoguang
刊名	ACS CHEMICAL BIOLOGY
	2015
关键词	BIOORTHOGONAL CHEMICAL REPORTERS GROWTH-FACTOR RECEPTOR FREE CLICK CHEMISTRY LIVING CELLS COPPER-FREE PENETRATING PEPTIDES ENZYMATIC-ACTIVITIES MAMMALIAN-CELLS QUINONE METHIDE CANCER-THERAPY
DOI	10.1021/acschembio.5b00193
英文摘要	Bioorthogonal ligations play a crucial role in labeling diverse types of biomolecules in living systems. Herein, we describe a novel class of ortho-quinolinone quinone methide (oQQM) precursors that show a faster kinetic rate in the click cycloaddition with thio-vinyl ether (TV) than the first generation TQ-ligation in both chemical and biological settings. We further demonstrate that the second generation TQ-ligation is also orthogonal to the widely used strain-promoted azide-alkyne cycloaddition (SPAAC) both in vitro and in vivo, revealing that these two types of bioorthogonal ligations could be used as an ideal reaction pair for the simultaneous tracking of multiple elements within a single system. Remarkably, the second generation TQ-ligation and SPAAC are effective for selective and simultaneous imaging of two different cell organelles in live cells.; National High Technology Projects 973 [2015CB856200, 2012CB837400]; NNSFC [21222209, 91313303, 21472010]; SCI(E); ARTICLE; xglei@pku.edu.cn; 7; 1676-1683; 10
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/418792]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Zhang, Xiaoyun,Dong, Ting,Li, Qiang,et al. Second Generation TQ-Ligation for Cell Organelle Imaging[J]. ACS CHEMICAL BIOLOGY,2015.
APA	Zhang, Xiaoyun.,Dong, Ting.,Li, Qiang.,Liu, Xiaohui.,Li, Lin.,...&Lei, Xiaoguang.(2015).Second Generation TQ-Ligation for Cell Organelle Imaging.ACS CHEMICAL BIOLOGY.
MLA	Zhang, Xiaoyun,et al."Second Generation TQ-Ligation for Cell Organelle Imaging".ACS CHEMICAL BIOLOGY (2015).