Regulation of DNA phosphorothioate modification in Salmonella enterica   by DndB

doi:10.1038/srep12368

CORC > 北京大学 > 化学与分子工程学院

	Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB
	He, Wei ; Huang, Teng ; Tang, You ; Liu, Yanhua ; Wu, Xiaolin ; Chen, Si ; Chan, Wan ; Wang, Yajie ; Liu, Xiaoyun ; Chen, Shi ; Wang, Lianrong
刊名	SCIENTIFIC REPORTS
	2015
关键词	STREPTOMYCES-LIVIDANS ESCHERICHIA-COLI MUTATIONAL ANALYSIS RESTRICTION SYSTEM S-MODIFICATION PROTEIN BACTERIA SULFUR REPAIR ACTIVATION
DOI	10.1038/srep12368
英文摘要	DNA phosphorothioate (PT) modification, in which the non-bridging oxygen of the sugar-phosphate backbone is substituted by sulfur, occurs naturally in diverse bacteria and archaea and is regulated by the DndABCDE proteins. DndABCDE and the restriction cognate DndFGHI constitute a new type of defense system that prevents the invasion of foreign DNA in Salmonella enterica serovar Cerro 87. GAAC/GTTC consensus contexts across genomes were found to possess partial PT modifications even in the presence of restriction activity, indicating the regulation of PT. The abundance of PT in cells must be controlled to suit cellular activities. However, the regulatory mechanism of PT modification has not been characterized. The result here indicated that genomic PT modification in S. enterica is controlled by the transcriptional regulator DndB, which binds to two regions in the promoter, each possessing a 5'-TACGN(10)CGTA-3' palindromic motif, to regulate the transcription of dndCDE and its own gene. Site-directed mutagenesis showed that the Cys29 residue of DndB plays a key role in its DNA-binding activity or conformation. Proteomic analysis identified changes to a number of cellular proteins upon up-regulation and loss of PT. Considering the genetic conservation of dnd operons, regulation of PT by DndB might be widespread in diverse organisms.; 973 program of Ministry of Science and Technology; National Science Foundation of China [31222001, 31130068, 31170049]; SCI(E); PubMed; ARTICLE; shichen@whu.edu.cn; lianrong@whu.edu.cn; 12368; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/418217]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	He, Wei,Huang, Teng,Tang, You,et al. Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB[J]. SCIENTIFIC REPORTS,2015.
APA	He, Wei.,Huang, Teng.,Tang, You.,Liu, Yanhua.,Wu, Xiaolin.,...&Wang, Lianrong.(2015).Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB.SCIENTIFIC REPORTS.
MLA	He, Wei,et al."Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB".SCIENTIFIC REPORTS (2015).