Biochemical and Structural Insights into the Mechanism of DNA   Recognition by Arabidopsis ETHYLENE INSENSITIVE3

doi:10.1371/journal.pone.0137439

CORC > 北京大学 > 化学与分子工程学院

	Biochemical and Structural Insights into the Mechanism of DNA Recognition by Arabidopsis ETHYLENE INSENSITIVE3
	Song, Jinghui ; Zhu, Chenxu ; Zhang, Xing ; Wen, Xing ; Liu, Lulu ; Peng, Jinying ; Guo, Hongwei ; Yi, Chengqi
刊名	PLOS ONE
	2015
关键词	RECEPTOR GENE FAMILY TRANSCRIPTION FACTOR SIGNAL-TRANSDUCTION RESPONSE PATHWAY EIN3 BINDING ETHYLENE-INSENSITIVE3 EXPRESSION REGULATOR JASMONATE
DOI	10.1371/journal.pone.0137439
英文摘要	Gaseous hormone ethylene regulates numerous stress responses and developmental adaptations in plants by controlling gene expression via transcription factors ETHYLENE INSENSITIVE3 (EIN3) and EIN3-Like1 (EIL1). However, our knowledge regarding to the accurate definition of DNA-binding domains (DBDs) within EIN3 and also the mechanism of specific DNA recognition by EIN3 is limited. Here, we identify EIN3 82-352 and 174-306 as the optimal and core DBDs, respectively. Results from systematic biochemical analyses reveal that both the number of EIN3-binding sites (EBSs) and the spacing length between two EBSs affect the binding affinity of EIN3; accordingly, a new DNA probe which has higher affinity with EIN3 than ERF1 is also designed. Furthermore, we show that palindromic repeat sequences in ERF1 promoter are not necessary for EIN3 binding. Finally, we provide, to our knowledge, the first crystal structure of EIN3 core DBD, which contains amino acid residues essential for DNA binding and signaling. Collectively, these data suggest the detailed mechanism of DNA recognition by EIN3 and provide an in-depth view at molecular level for the transcriptional regulation mediated by EIN3.; National Basic Research Foundation of China [2014CB964900]; National Natural Science Foundation of China [31270838, 21472009]; SCI(E); PubMed; ARTICLE; hongweig@pku.edu.cn; chengqi.yi@pku.edu.cn; 9; e0137439; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/416337]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Song, Jinghui,Zhu, Chenxu,Zhang, Xing,et al. Biochemical and Structural Insights into the Mechanism of DNA Recognition by Arabidopsis ETHYLENE INSENSITIVE3[J]. PLOS ONE,2015.
APA	Song, Jinghui.,Zhu, Chenxu.,Zhang, Xing.,Wen, Xing.,Liu, Lulu.,...&Yi, Chengqi.(2015).Biochemical and Structural Insights into the Mechanism of DNA Recognition by Arabidopsis ETHYLENE INSENSITIVE3.PLOS ONE.
MLA	Song, Jinghui,et al."Biochemical and Structural Insights into the Mechanism of DNA Recognition by Arabidopsis ETHYLENE INSENSITIVE3".PLOS ONE (2015).