Single-wall carbon nanohorns inhibited activation of microglia induced   by lipopolysaccharide through blocking of Sirt3

doi:10.1186/1556-276X-8-100

CORC > 北京大学 > 化学与分子工程学院

	Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3
	Li, Lihong ; Zhang, Jinqian ; Yang, Yang ; Wang, Qiang ; Gao, Li ; Yang, Yanlong ; Chang, Tao ; Zhang, Xingye ; Xiang, Guoan ; Cao, Yongmei ; Shi, Zujin ; Zhao, Ming ; Gao, Guodong
刊名	nanoscale research letters
	2013
关键词	Single-walled carbon nanohorns Septic encephalopathy Cell proliferation Apoptosis Lipopolysaccharide Sepsis Sirt3 MS/MS PROTEOME ANALYSIS HEPATOMA HEPG2 CELLS IN-VITRO TOXICITY NAD(+) DEPLETION OXIDATIVE STRESS MACROPHAGE CELLS DRUG CARRIERS NANOTUBES CYTOTOXICITY DEATH
DOI	10.1186/1556-276X-8-100
英文摘要	Single-wall carbon nanohorns (SWNHs) have been demonstrated to accumulate in cytotoxic levels within organs of various animal models and cell types, which emerge as a wide range of promising biomedical imaging. Septic encephalopathy (SE) is an early sign of sepsis and associated with an increased rate of morbidity and mortality. Microglia activation plays an important role in neuroinflammation, which contributes to neuronal damage. Inhibition of microglia activation may have therapeutic benefits, which can alleviate the progression of neurodegeneration. Therefore, we investigated the functional changes of mice microglia cell lines pre-treated with or without lipopolysaccharide (LPS) induced by SWNHs. To address this question, the research about direct role of SWNHs on the growth, proliferation, and apoptosis of microglia cell lines in mice (N9 and BV2) pre-treated with or without LPS had been performed. Our results indicate that the particle diameter of SWNHs in water is between 342 to 712 nm. The images in scanning electron microscope showed that SWNHs on polystyrene surface are individual particles. LPS induced activation of mice microglia, promoted its growth and proliferation, and inhibited its apoptosis. SWNHs inhibited proliferation, delayed mitotic entry, and promoted apoptosis of mice microglia cells. The effects followed gradually increasing cultured time and concentrations of SWNHs, especially in cells pre-treated with LPS. SWNHs induced a significantly increase in G1 phase and inhibition of S phase of mice microglia cells in a dose-manner dependent of SWNHs, especially in cells pre-treated with LPS. The transmission electron microscope images showed that individual spherical SWNH particles smaller than 100 nm in diameters were localized inside lysosomes of mice microglia cells. SWNHs inhibited mitotic entry, growth and proliferation of mice microglia cells, and promoted its apoptosis, especially in cells pre-treated with LPS. SWNHs inhibited expression of Sirt3 and energy metabolism related with Sirt3 in mice microglia cells in a dose-dependent manner, especially in cells pre-treated with LPS. The role of SWNHs on mice microglia was implicating Sirt3 and energy metabolism associated with it.; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; SCI(E); EI; 3; ARTICLE; 1; 1-13; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/390767]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Lihong,Zhang, Jinqian,Yang, Yang,et al. Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3[J]. nanoscale research letters,2013.
APA	Li, Lihong.,Zhang, Jinqian.,Yang, Yang.,Wang, Qiang.,Gao, Li.,...&Gao, Guodong.(2013).Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3.nanoscale research letters.
MLA	Li, Lihong,et al."Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3".nanoscale research letters (2013).