Determination of estrodiol by capillary electrophoretic immunoassay with   thermally reversible hydrogel

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	Determination of estrodiol by capillary electrophoretic immunoassay with thermally reversible hydrogel
	Su, P ; Wang, YC ; Zhang, XX ; Sun, L ; Chang, WB
刊名	高等学校化学学报
	2000
关键词	hydrogel capillary electrophoresis laser-induced fluorescence immunoassay estradiol ENZYME-IMMUNOASSAY ESTRADIOL POLYMERS SERUM ASSAY
英文摘要	In this paper, a kind of thermally linear polymer, poly-N-isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can he used to separate bound and free estradiol antigen, A useful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay (CEIA) was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mL and 0. 1-10 ng/mL.; Chemistry, Multidisciplinary; SCI(E); EI; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 5; 6; 870-872; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389445]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Su, P,Wang, YC,Zhang, XX,et al. Determination of estrodiol by capillary electrophoretic immunoassay with thermally reversible hydrogel[J]. 高等学校化学学报,2000.
APA	Su, P,Wang, YC,Zhang, XX,Sun, L,&Chang, WB.(2000).Determination of estrodiol by capillary electrophoretic immunoassay with thermally reversible hydrogel.高等学校化学学报.
MLA	Su, P,et al."Determination of estrodiol by capillary electrophoretic immunoassay with thermally reversible hydrogel".高等学校化学学报 (2000).