FLOW-INJECTION AND LIQUID-CHROMATOGRAPHIC POSTCOLUMN DETECTION OF   AMINO-ACIDS BY MIMETIC PEROXIDASE-CATALYZED CHEMILUMINESCENCE REACTION

doi:10.1016/0003-2670(92)85139-W

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	FLOW-INJECTION AND LIQUID-CHROMATOGRAPHIC POSTCOLUMN DETECTION OF AMINO-ACIDS BY MIMETIC PEROXIDASE-CATALYZED CHEMILUMINESCENCE REACTION
	CI, YX ; TIE, JK ; WANG, QW ; CHANG, WB
刊名	analytica chimica acta
	1992
关键词	CHEMILUMINESCENCE FLOW INJECTION LIQUID CHROMATOGRAPHY AMINO ACIDS METALLOPORPHYRINS HYDROGEN-PEROXIDE HOMOVANILLIC-ACID METALLOPORPHYRINS LUMINOL
DOI	10.1016/0003-2670(92)85139-W
英文摘要	Four amino acids were determined on the basis of the finding that the catalytic activity of mimetic peroxidase (metalloporphyrin) in the chemiluminescence reaction between luminol and hydrogen peroxide is inhibited in the presence of an amino acid. The degree of chemiluminescence inhibition is a measure of the amino acid concentration. The electrostatic interaction between amino acid and metalloporphyrin was confirmed by comparing the degree of inhibition of cationic and anionic metalloporphyrin-catalysed chemiluminescence reactions. More than 20 amino acids were tested, and only L-cysteine, L-tyrosine, L-tryptophan and L-cystine significantly quenched the chemiluminescence intensity. Detection limits were 6.8 X 10(-8), 1.3 X 10(-7), 8.5 X 10(-6) and 2.2 X 10(-5) M, respectively. The detection approach is demonstrated with a silica-based liquid chromatographic separation of amino acids using phosphate buffer (pH 7.3) as mobile phase. Compared with other chemiluminescence analyses, this method is faster, can be run at room temperature and, in favourable cases, has a lower detection limit.; Chemistry, Analytical; SCI(E); 20; ARTICLE; 1; 109-114; 269
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/313534]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	CI, YX,TIE, JK,WANG, QW,et al. FLOW-INJECTION AND LIQUID-CHROMATOGRAPHIC POSTCOLUMN DETECTION OF AMINO-ACIDS BY MIMETIC PEROXIDASE-CATALYZED CHEMILUMINESCENCE REACTION[J]. analytica chimica acta,1992.
APA	CI, YX,TIE, JK,WANG, QW,&CHANG, WB.(1992).FLOW-INJECTION AND LIQUID-CHROMATOGRAPHIC POSTCOLUMN DETECTION OF AMINO-ACIDS BY MIMETIC PEROXIDASE-CATALYZED CHEMILUMINESCENCE REACTION.analytica chimica acta.
MLA	CI, YX,et al."FLOW-INJECTION AND LIQUID-CHROMATOGRAPHIC POSTCOLUMN DETECTION OF AMINO-ACIDS BY MIMETIC PEROXIDASE-CATALYZED CHEMILUMINESCENCE REACTION".analytica chimica acta (1992).