Direct Observation of the Uptake of Outer Membrane Proteins by the   Periplasmic Chaperone Skp

doi:10.1371/journal.pone.0046068

CORC > 北京大学 > 化学与分子工程学院

	Direct Observation of the Uptake of Outer Membrane Proteins by the Periplasmic Chaperone Skp
	Lyu, Zhi-Xin ; Shao, Qiang ; Gao, Yi Qin ; Zhao, Xin Sheng
刊名	plos one
	2012
关键词	FLUORESCENCE CORRELATION SPECTROSCOPY ESCHERICHIA-COLI CRYSTAL-STRUCTURE BIOGENESIS DYNAMICS AGGREGATION COMPLEXES MOLECULES MECHANISM EVOLUTION
DOI	10.1371/journal.pone.0046068
英文摘要	The transportation of membrane proteins through the aqueous subcellular space is an important and challenging process. Its molecular mechanism and the associated structural change are poorly understood. Periplasmic chaperones, such as Skp in Escherichia coli, play key roles in the transportation and protection of outer membrane proteins (OMPs) in Gram-negative bacteria. The molecular mechanism through which Skp interacts with and protects OMPs remains mysterious. Here, a combined experimental and molecular dynamics simulation study was performed to gain the structural and dynamical information in the process of OMPs and Skp binding. Stopped-flow experiments on site specific mutated and labeled Skp and several OMPs, namely OmpC, the transmembrane domain of OmpA, and OmpF, allowed us to obtain the mechanism of OMP entering the Skp cavity, and molecular dynamics simulations yielded detailed molecular interactions responsible for this process. Both experiment and simulation show that the entrance of OMP into Skp is a highly directional process, which is initiated by the interaction between the N-terminus of OMP and the bottom "tentacle" domain of Skp. The opening of the more flexible tentacle of Skp, the non-specific electrostatic interactions between OMP and Skp, and the constant formation and breaking of salt bridges between Skp and its substrate together allow OMP to enter Skp and gradually "climb" into the Skp cavity in the absence of an external energy supply.; Multidisciplinary Sciences; SCI(E); 4; ARTICLE; 9; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/313076]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Lyu, Zhi-Xin,Shao, Qiang,Gao, Yi Qin,et al. Direct Observation of the Uptake of Outer Membrane Proteins by the Periplasmic Chaperone Skp[J]. plos one,2012.
APA	Lyu, Zhi-Xin,Shao, Qiang,Gao, Yi Qin,&Zhao, Xin Sheng.(2012).Direct Observation of the Uptake of Outer Membrane Proteins by the Periplasmic Chaperone Skp.plos one.
MLA	Lyu, Zhi-Xin,et al."Direct Observation of the Uptake of Outer Membrane Proteins by the Periplasmic Chaperone Skp".plos one (2012).