Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal   the Upregulation of Sialylation during Cardiac Hypertrophy

doi:10.1021/ja508484c

CORC > 北京大学 > 化学与分子工程学院

	Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy
	Rong, Jie ; Han, Jing ; Dong, Lu ; Tan, Yanhong ; Yang, Huaqian ; Feng, Lianshun ; Wang, Qi-Wei ; Meng, Rong ; Zhao, Jing ; Wang, Shi-Qiang ; Chen, Xing
刊名	journal of the american chemical society
	2014
关键词	O-LINKED GLYCOSYLATION FREE CLICK CHEMISTRY LIVING ANIMALS COPPER-FREE CARDIOMYOCYTE HYPERTROPHY VENTRICULAR MYOCYTES MASS-SPECTROMETRY MODIFIED PROTEINS FAILING HEART CELL-SURFACES
DOI	10.1021/ja508484c
英文摘要	In the heart, glycosylation is involved in a variety of physiological and pathological processes. Cardiac glycosylation is dynamically regulated, which remains challenging to monitor in vivo. Here we describe a chemical approach for analyzing the dynamic cardiac glycome by metabolically labeling the cardiac glycans with azidosugars in living rats. The azides, serving as a chemical reporter, are chemoselectively conjugated with fluorophores using copper-free click chemistry for glycan imaging; derivatizing azides with affinity tags allows enrichment and proteomic identification of glycosylated cardiac proteins. We demonstrated this methodology by visualization of the cardiac sialylated glycans in intact hearts and identification of more than 200 cardiac proteins modified with sialic acids. We further applied this methodology to investigate the sialylation in hypertrophic hearts. The imaging results revealed an increase of sialic acid biosynthesis upon the induction of cardiac hypertrophy. Quantitative proteomic analysis identified multiple sialylated proteins including neural cell adhesion molecule 1, T-kininogens, and alpha(2)-macroglobulin that were upregulated during hypertrophy. The methodology may be further extended to other types of glycosylation, as exemplified by the mucin-type O-linked glycosylation. Our results highlight the applications of metabolic glycan labeling coupled with bioorthogonal chemistry in probing the biosynthesis and function of cardiac glycome during pathophysiological responses.; Chemistry, Multidisciplinary; SCI(E); EI; 1; ARTICLE; jingzhao@nju.edu.cn; wsq@pku.edu.cn; xingchen@pku.edu.cn; 50; 17468-17476; 136
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/312673]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Rong, Jie,Han, Jing,Dong, Lu,et al. Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy[J]. journal of the american chemical society,2014.
APA	Rong, Jie.,Han, Jing.,Dong, Lu.,Tan, Yanhong.,Yang, Huaqian.,...&Chen, Xing.(2014).Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy.journal of the american chemical society.
MLA	Rong, Jie,et al."Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy".journal of the american chemical society (2014).