固绿FCF分光光度法测定血清蛋白质

doi:10.3321/j.issn:0253-3820.2003.12.008

CORC > 北京大学 > 化学与分子工程学院

	固绿FCF分光光度法测定血清蛋白质; Spectrophotometric Determination of Serum Proteins with Fast Green FCF
	马卫兴 ; 钱保华 ; 杨绪杰 ; 陆路德 ; 汪信 ; 李克安
刊名	分析化学
	2003
关键词	固绿FCF 分光光度法蛋白质 fast green FCF spectrophotometry proteins
DOI	10.3321/j.issn:0253-3820.2003.12.008
英文摘要	在pH 1.4的Clark-Lubs 缓冲介质中,固绿FCF与血清蛋白质作用在室温下能迅速结合形成复合物,其最大吸收波长为660 nm,比固绿FCF本身红移了36 nm.用分光光度法研究了该结合反应的最佳条件,并在此基础上建立了测定蛋白质的新方法.在660 nm处各蛋白质浓度至少在5～70 mg/L的范围内与吸光度成正比,对测定牛血清白蛋白(BSA)、人血清白蛋白(HSA)及球蛋白(IgG)的表观摩尔吸光系数ε660和桑德尔灵敏度s分别为7.87×105、9.52×105、1.60×106 Lmol-1cm-1和0.083、0.072、0.093 μg*cm-2.除阴、阳离子表面活性剂外,其余大部分物质不干扰蛋白质的测定.所拟方法具有简便、快速、选择性好、灵敏度高等特点,应用于尿液、人血清及含乳饮料中总蛋白的测定,结果与考马斯亮蓝G-250法基本一致.; In the Clark-Lubs medium with pH 1.4, fast green FCF can bind rapidly to form a complex with serum protein at room temperature, which has a maximum absorption wavelength at 660 nm, and exhibits a bathochromic shift of 36 nm compared with the maximum absorption wavelength of fast green FCF. The optimum reaction conditions were investigated, and a new method for the determination of proteins was developed based on this binding reaction. The absorbance of the binding system at 660 nm is proportional to the concentration of some proteins in the range of 5similar to70 mg/L with a molar absorptivity of 7.87x10(5), 9.52x10(5), 1.60x10(6) L.mol(-1).cm(-1) and Sandell sensitivity of 0.083, 0.072, 0.093 mug/cm(-2) for the determination of bovine serum albumin (BSA), human serum albumin (HAS) and human immunoglobulin G (IgG), respectively. A lot of the foreign substances do not interfere with the determination of proteins except for the anionic surfactant and cationic surfactant. The proposed method, which is rapid, stable, and of good selectivity and high sensitivity, has been used for the determination of the total proteins in urine and human serum samples as well as milk drink samples. The results obtained by this method agreed with those obtained by the coomassie brillant blue G-250 method.; 江苏省教育厅自然科学基金; 淮海工学院校科研和教改项目; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 1; 12; 1437-1441; 31
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/255780]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	马卫兴,钱保华,杨绪杰,等. 固绿FCF分光光度法测定血清蛋白质, Spectrophotometric Determination of Serum Proteins with Fast Green FCF[J]. 分析化学,2003.
APA	马卫兴,钱保华,杨绪杰,陆路德,汪信,&李克安.(2003).固绿FCF分光光度法测定血清蛋白质.分析化学.
MLA	马卫兴,et al."固绿FCF分光光度法测定血清蛋白质".分析化学 (2003).